Quantification of Pathogenicity Genes Expression in Pectobacterium carotovorum by qRT-PCR
Elfarash, A., Abo Elyousr, K., Morsy, Z., Amein, K. (2021). Quantification of Pathogenicity Genes Expression in Pectobacterium carotovorum by qRT-PCR. EKB Journal Management System, 12(3), 55-59. doi: 10.21608/jacb.2021.66184.1012
A. E. Elfarash; K. A. Abo Elyousr; Zynab S. Morsy; K. A. Amein. "Quantification of Pathogenicity Genes Expression in Pectobacterium carotovorum by qRT-PCR". EKB Journal Management System, 12, 3, 2021, 55-59. doi: 10.21608/jacb.2021.66184.1012
Elfarash, A., Abo Elyousr, K., Morsy, Z., Amein, K. (2021). 'Quantification of Pathogenicity Genes Expression in Pectobacterium carotovorum by qRT-PCR', EKB Journal Management System, 12(3), pp. 55-59. doi: 10.21608/jacb.2021.66184.1012
Elfarash, A., Abo Elyousr, K., Morsy, Z., Amein, K. Quantification of Pathogenicity Genes Expression in Pectobacterium carotovorum by qRT-PCR. EKB Journal Management System, 2021; 12(3): 55-59. doi: 10.21608/jacb.2021.66184.1012

Quantification of Pathogenicity Genes Expression in Pectobacterium carotovorum by qRT-PCR

Journal of Agricultural Chemistry and Biotechnology
Article 2, Volume 12, Issue 3, March 2021, Page 55-59  XML PDF (608.55 K)
Document Type: Original Article
DOI: 10.21608/jacb.2021.66184.1012
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Authors
A. E. Elfarash email 1; K. A. Abo Elyousr2; Zynab S. Morsy3; K. A. Amein1
1Department of Genetics, Faculty of Agriculture, Assiut University, Assiut, Egypt.
2Department of Plant Pathology, Faculty of Agriculture, Assiut University, Assiut, Egypt.
3Central laboratories, Faculty of Agriculture, Assiut University, Assiut, Egypt.
Abstract
Bacterial soft rot disease is one of the most important and widespread bacterial diseases that cause destructive losses to the crop in a matter of days. The disease causes severe many vegetable crops including potato. In this study, four isolates of Pectobacterium carotovorum the main causal of the soft rot disease were isolated from naturally rotted tubers. Then, the isolates were subjected to a new set of primer pairs designed to amplify the pel gene which distinguish the P. carotovorum  strains. The results confirmed that four isolates belong to P. carotovorum  by having the pel gene. Then, a pathogenicity test was performed on potato tubers which showed variability between the isolates in pathogenicity and diseases severity. Finally, a quantitative reverse transcriptase PCR (qRT-PCR) techniquewas used to investigate the effect of the presence of the potatoes tissues on stimulating the expression of the pathogenicity genes. The gene expression of five pathogenicity genes was studied included: polygalacturonase (Peh), pectate lyase (Pel), xylanase (Xy), protease (Prt), and cellulase (Cel). The results confirmed that the presence of potatoes tissues stimulates the expression of pathogenicity genes in both strong and weak P. carotovorum  isolates.
Keywords
P. carotovorum; qRT-PCR; Polygalacturonase; pectate lyase – cellulase; xylanase; protease
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