Role of Carboxyamidase in the in vivo metabolism of Chlorfluazuron in the Black Cutworm, Agrotis ipsilon (Hfn.) (Lepidoptera : Noctuidae) | ||||
| Egyptian Academic Journal of Biological Sciences. A, Entomology | ||||
| Article 6, Volume 1, Issue 2, December 2008, Page 65-70 PDF (331.62 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/eajbsa.2008.15733 | ||||
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| Author | ||||
| Adel Ramzy Fahmy | ||||
| Entomology Department, Faculty of Science, Ain Shams University Cairo 11566, Egypt | ||||
| Abstract | ||||
| Triphenyl phosphate (TPP), a carboxylesterase inhibitor, has synergized chlorfluazuron in the resistant strain of the black cutworm but not in the susceptible strain. In vivo metabolism of 14C – chlorfluazuron revealed that the amount of radiolabeled major metabolites (2,6 – difluorobenzoic acid and 2,6 – difluorobenzamide) recovered from resistant larvae was four times higher than susceptible larvae. Addition of TPP did not affect chlorfluazuron metabolism pattern in the susceptible insects but it caused a significant reduction of the amount of detected metabolites in the resistant insects. Cleavage of chlorfluazuron molecule took place at one or both of the urea C-NH-C bonds which suggests the responsibility of carboxyamidase enzyme for chlorfluazuron metabolism. In vitro assays revealed that carboxyamidase activity was about four times higher in the resistant larvae than the susceptible ones which supports the hypothesis of the involvement of carboxyamidase in chlorfluazuron resistance in this insect pest. | ||||
| Keywords | ||||
| black cutworm – chlorfluazuron – in vivo degradation – carboxyamidase | ||||
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