Prevalence of enterotoxigenic Staphylococcus aureus with characterization of enterotoxins for subsequent detection
Mohamed, F., H, O., Deif, H., Ragab, E., Eljakee, J. (2020). Prevalence of enterotoxigenic Staphylococcus aureus with characterization of enterotoxins for subsequent detection. EKB Journal Management System, 66(1), 98-114. doi: 10.21608/vmjg.2020.157909
Fatma Elzahraa Mohamed; Omnia H; Heba Deif; Eman Ragab; Jakeen Eljakee. "Prevalence of enterotoxigenic Staphylococcus aureus with characterization of enterotoxins for subsequent detection". EKB Journal Management System, 66, 1, 2020, 98-114. doi: 10.21608/vmjg.2020.157909
Mohamed, F., H, O., Deif, H., Ragab, E., Eljakee, J. (2020). 'Prevalence of enterotoxigenic Staphylococcus aureus with characterization of enterotoxins for subsequent detection', EKB Journal Management System, 66(1), pp. 98-114. doi: 10.21608/vmjg.2020.157909
Mohamed, F., H, O., Deif, H., Ragab, E., Eljakee, J. Prevalence of enterotoxigenic Staphylococcus aureus with characterization of enterotoxins for subsequent detection. EKB Journal Management System, 2020; 66(1): 98-114. doi: 10.21608/vmjg.2020.157909

Prevalence of enterotoxigenic Staphylococcus aureus with characterization of enterotoxins for subsequent detection

Veterinary Medical Journal (Giza)
Article 9, Volume 66, Issue 1, 2020, Page 98-114  XML PDF (474.54 K)
Document Type: Original Article
DOI: 10.21608/vmjg.2020.157909
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Authors
Fatma Elzahraa Mohamed1; Omnia H2; Heba Deif1; Eman Ragab1; Jakeen Eljakee* 1
1Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Giza.
2Animal Health Research institute, Giza, Egypt.
Abstract
In this work 68 S. aureus isolated frommastitic cows (n=48), camel milk (n=5) and human samples (urine n = 4, abscesses aspiration n = 3, sputum n = 2, nasal swabs n = 2, vaginal swabs n = 1 and wound swabs n = 3) were investigated using phenotypic and molecular identification to detect the occurrence of enterotoxins. Eighteen out of 48 S. aureus isolated from mastitic cows and 5 out of 15 S. aureus isolated from human samples were enterotoxin producers. All toxigenic S. aureus isolated from clinical mastitic cows (13) were toxin type D producer while seb and sed were detected from subclinical mastitic cows. Among human isolates, 2 isolates produced mixed toxins A and E, 2 isolates produced mixed toxins C and D and one isolate produced type C toxin. No toxin was detected from camel isolates. All toxigenic S. aureus isolated from cows (13) were toxin type D producer. Among human isolates 2 isolates produced mixed toxins A and E, 2 isolates produced mixed toxins C & D and one isolate produced type C toxin.  Five S. aureuscrude enterotoxin type D were investigated by protein SDS-PAGE. It was clear that all isolates had from 9 to 7 bands.  All the isolates had a band at 148-159 kDa, 35-42 kDa, 28-30 kDa, 23-26 kDa, 20-22 kDa and 18-19 kDa. 4 isolates had a band at 48-55 kDa, 3 isolates had a band at 39-42 kDa, 3 isolates had a band at 31-34 kDa and one isolate had a band at 17 kDa.
Keywords
Enterotoxin; Multiplex PCR; Mastitic milk; S. aureus; SDS
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