The Possible Protective Effect of Magnolol on Triolein-Induced Lung Structural Changes in Rats: Histological and Immunohistochemical Study | ||||
| Egyptian Journal of Histology | ||||
| Article 3, Volume 45, Issue 2, June 2022, Page 359-371 PDF (4.05 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2021.62560.1429 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Samah Kandeel   1; Remon S. Estfanous2
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| 1Histology Department, Faculty of Medicine, Tanta University | ||||
| 2Anatomy and Embryology department, Faculty of Medicine, Tanta University, Egypt | ||||
| Abstract | ||||
| Introduction: Triolein is a neutral fat derives from an oleic acid. It has been used as a textile lubricant, a plasticizer and present in cocoa butter. Its occupational exposure lead to health hazards especially lung injury. Magnolol is a Chinese herbal complex with anti-inflammatory, antioxidant and antifibrotic actions. Aim: The present research aimed to evaluate the possible protective effect of magnolol on triolein-induced lung structural changes in rats using histological and immunohistochemical study. Materials and Methods: 45 male Wistar rats were used. Their weights were from 120 to 150 gm. They were divided into Group 1 (15 rats as control group); Group 2: 15 rats that received 0.2 ml triolein at the caudal vein then were sacrificed after 2, 4 and 21 days; Group 3: 15 rats that received 50 mg/kg oral dose of magnolol daily 60 minutes before giving triolein then were sacrificed after 2, 4 and 21 days. Results: Group 2 showed significant thickened inter-alveolar septa, inflammatory cellular infiltrations, congested blood vessels, luminal inflammatory exudates, disorganized sloughed epithelium and lost cilia at H&E stained sections. Group 2 also showed significantly increased deposition of collagen fibers, significantly increased expression of alpha –Smooth muscle actin (α-SMA) in the interalveolar septa, wall of the blood vessels and bronchioles and significant increase in the number of macrophages with positive CD68 (Cluster of Differentiation 68) immunohistochemical reaction. Group 3 showed improvements of the previous pathological findings. Conclusion: Magnolol could protect the lung from the injurious effect of triolein possibly due to its anti-inflammatory, antioxidant and antifibrotic actions. This may favor the use of magnolol for the treatment of lung injuries. | ||||
| Keywords | ||||
| Histology; immunohistochemistry; lung; magnolol; triolein | ||||
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