Efficacy of Aqueous Extract of Saffron (Crocus sativus L.) in Modulating Radiation-Induced Brain and Eye Retina Damage in Rats
A. Sh, A., N.H., S., N. A, E. (2014). Efficacy of Aqueous Extract of Saffron (Crocus sativus L.) in Modulating Radiation-Induced Brain and Eye Retina Damage in Rats. EKB Journal Management System, 54(1), 101-108. doi: 10.12816/0002436
Abd El-Azime A. Sh; Sherif N.H.; Eltahawy N. A. "Efficacy of Aqueous Extract of Saffron (Crocus sativus L.) in Modulating Radiation-Induced Brain and Eye Retina Damage in Rats". EKB Journal Management System, 54, 1, 2014, 101-108. doi: 10.12816/0002436
A. Sh, A., N.H., S., N. A, E. (2014). 'Efficacy of Aqueous Extract of Saffron (Crocus sativus L.) in Modulating Radiation-Induced Brain and Eye Retina Damage in Rats', EKB Journal Management System, 54(1), pp. 101-108. doi: 10.12816/0002436
A. Sh, A., N.H., S., N. A, E. Efficacy of Aqueous Extract of Saffron (Crocus sativus L.) in Modulating Radiation-Induced Brain and Eye Retina Damage in Rats. EKB Journal Management System, 2014; 54(1): 101-108. doi: 10.12816/0002436

Efficacy of Aqueous Extract of Saffron (Crocus sativus L.) in Modulating Radiation-Induced Brain and Eye Retina Damage in Rats

The Egyptian Journal of Hospital Medicine
Article 13, Volume 54, Issue 1, January 2014, Page 101-108  XML PDF (404.54 K)
Document Type: Original Article
DOI: 10.12816/0002436
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Authors
Abd El-Azime A. Sh1; Sherif N.H.2; Eltahawy N. A3
1Radiation Biology Departmen National Centre for Radiation Research and Technology (NCRRT), Atomic Energy Authority(AEA) , Cairo, Egypt.
2Drug radiation research Department National Centre for Radiation Research and Technology (NCRRT), Atomic Energy Authority(AEA) , Cairo, Egypt.
3Faculty of Applied Medical Science, Misr University for Science and Technology
Abstract
Background: Saffron (Crocus sativusL.)is a plant of the iris family (Iridaceae). Its stigma contains crocin, anthocyanin, carotene and lycopene which are known to have pharmacological effects on various illnesses. The aim of present study was to investigate the role of aqueous extract of saffron on the radiation-induced changes in rat (eye retina, brain) tissues and blood. Material & methods: Saffron was supplemented orally, via gavages to rats at dose of 100 mg/Kg body wt/day for 2 weeks pre-exposure to 6.5 Gy (one shot dose) of whole body gamma-irradiation. Animals were sacrificed at the 1st day post radiation exposure.
Results: whole body gamma irradiation of rats induce oxidative stress in eye retina and brain tissues and blood identified by significant elevation in the level of malondialdhyde (MDA), advanced oxidation protein products (AOPP) and protein carbonyl(PC) contents associated with significant decreases of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (Gpx) activities, and glutathione (GSH) content. Oxidative stress was concomitant with a significant decrease in brain dopamine (DA), norepinephrine (NE) and epinephrine (EPI) contents. Supplemented with Saffron extract pre- irradiation have significantly reduced the severity of radiation-induced oxidative stress and catecholamines alteration in the organs under investigations.
Conclusion: Saffron exerts its modulating effect in the organs under investigations due to the presence of associated bioactive compounds with antioxidant properties.         
 
Keywords
Saffron; eye retina; Brain; Gamma rays; Blood; Rats; Lipid peroxidation; Oxidative Stress
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