Screening of Occult Hepatitis B Virus Infection among Egyptian Blood Donors.
Attia, A., Elmetwalli, A. (2021). Screening of Occult Hepatitis B Virus Infection among Egyptian Blood Donors.. EKB Journal Management System, 5.2(2), 27-31. doi: 10.21608/mjvh.2021.158276
Attia Ahmed Attia; Alaa Elmetwalli. "Screening of Occult Hepatitis B Virus Infection among Egyptian Blood Donors.". EKB Journal Management System, 5.2, 2, 2021, 27-31. doi: 10.21608/mjvh.2021.158276
Attia, A., Elmetwalli, A. (2021). 'Screening of Occult Hepatitis B Virus Infection among Egyptian Blood Donors.', EKB Journal Management System, 5.2(2), pp. 27-31. doi: 10.21608/mjvh.2021.158276
Attia, A., Elmetwalli, A. Screening of Occult Hepatitis B Virus Infection among Egyptian Blood Donors.. EKB Journal Management System, 2021; 5.2(2): 27-31. doi: 10.21608/mjvh.2021.158276

Screening of Occult Hepatitis B Virus Infection among Egyptian Blood Donors.

Medical Journal of Viral Hepatitis
Article 6, Volume 5.2, Issue 2, March 2021, Page 27-31  XML PDF (144.33 K)
Document Type: Original article
DOI: 10.21608/mjvh.2021.158276
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Authors
Attia Ahmed Attia email 1; Alaa Elmetwalliorcid 2
1Department of Microbiology, Faculty of Science, Benha University, Benha, Egypt.
2Department of Clinical Trial Research Unit, Egyptian Liver Research Institute and Hospital, Mansoura, Egypt.
Abstract
Background:Although sensitive screening assays for hepatitis B virus surface antigen (HBsAg) are available, special cases of post-transfusion hepatitis B virus infection still occur. The present study was conducted to evaluate the prevalence of anti-hepatitis B core (anti-HBc) positivity and the presence of HBV-DNA in serum samples of healthy blood donors negative for both HBsAg and anti-HCV antibodies in Benha, Egypt.
Materials and methods: The study included a screening of 450 selected blood donors. The distribution of blood donors was 288 males (64%) and 162 females (36%). The recruited blood donors who met the criteria for blood donation were routinely screened for HBsAg, HIV I/2-Ab, and syphilis-antibodies. The blood units for donations were further analyzed for the presence of HBc-IgM and HBV-DNA levels by PCR method.
Results: Testing of the accepted units for the donation was about 12 (2.7%) HBc-IgM positive, and 9 (2%) HBV-DNA positive units. The standard screening of blood unit failed to recognize early acute or window HBV infection where HBsAg is missing.
Conclusions:Our investigations proposed that sensitive methods for the detection of HBV by PCR might be recommended in the screening of donated blood. Furthermore, anti-HBc antibodies should be tested regularly on all blood donation units.
Keywords
Hepatitis B virus; blood transfusion; occult infection; polymerase chain reaction
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