Association between mutations in GYR A/B and PAR C/E genes of Acinetobacter Baumannii clinical isolates and Fluoroquinolones resistance
Elshahat, M., Aboelized, E., Abd El Rahman, R., Nabil, M., Yassin, A. (2021). Association between mutations in GYR A/B and PAR C/E genes of Acinetobacter Baumannii clinical isolates and Fluoroquinolones resistance. EKB Journal Management System, 2(2), 343-351. doi: 10.21608/mid.2021.64414.1127
Mohamed Elshahat; Esam Aboelized; Raafat Abd El Rahman; Mohamed Nabil; Ayman Yassin. "Association between mutations in GYR A/B and PAR C/E genes of Acinetobacter Baumannii clinical isolates and Fluoroquinolones resistance". EKB Journal Management System, 2, 2, 2021, 343-351. doi: 10.21608/mid.2021.64414.1127
Elshahat, M., Aboelized, E., Abd El Rahman, R., Nabil, M., Yassin, A. (2021). 'Association between mutations in GYR A/B and PAR C/E genes of Acinetobacter Baumannii clinical isolates and Fluoroquinolones resistance', EKB Journal Management System, 2(2), pp. 343-351. doi: 10.21608/mid.2021.64414.1127
Elshahat, M., Aboelized, E., Abd El Rahman, R., Nabil, M., Yassin, A. Association between mutations in GYR A/B and PAR C/E genes of Acinetobacter Baumannii clinical isolates and Fluoroquinolones resistance. EKB Journal Management System, 2021; 2(2): 343-351. doi: 10.21608/mid.2021.64414.1127

Association between mutations in GYR A/B and PAR C/E genes of Acinetobacter Baumannii clinical isolates and Fluoroquinolones resistance

Microbes and Infectious Diseases
Article 20, Volume 2, Issue 2, May 2021, Page 343-351  XML PDF (409.55 K)
Document Type: Original Article
DOI: 10.21608/mid.2021.64414.1127
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Authors
Mohamed Elshahat1; Esam Aboelized1; Raafat Abd El Rahman2; Mohamed Nabil3; Ayman Yassin email 3
1Department of Microbiology and Immunology, Faculty of Medicine, Al-Azhar University, Cairo, Egypt
2Department of Microbiology and Immunology, Military Medical Academy, Cairo, Egypt
3Department of Microbiology and Immunology, Faculty of Medicine, Al-Azhar University, Assuit, Egypt
Abstract
Background: Acinetobacter baumannii (A. baumannii) is an important organism in hospital acquired infections. A. baumannii has emerged as Quinolones resistant organism.The maximum crucial mechanism of fluoroquinolones resistance is the mutation in genes that encode the subunits of DNA gyrase (gyrA and gyrB) and topoisomerase IV (parC and parE). Objectives: determination of mutations of gyrA/B and parC/E genes using Restriction Fragment Length Polymorphism PCR (RFLP - PCR) among fluoroquinolones resistant clinical isolates of A. baumannii showed in ICU patients at Al-Azhar University hospitals. Subjects & Methods: The study was conducted on 100 isolates of A.baumannii which subjected to molecular study of mutations of gyrA, gyrB, parC and parE genes beside determination of minimal inhibitory concentration (MIC). Results: The most A. baumannii isolates had MIC >128 µg/ml (61.1%). The resistant isolates showed combind mutations in both gyrA& parC genes most frequently (79.2%) then gyrA (9.7%), parC (8.3%) and gyrB (2.8%). Conclusion: Mutations in gyrA and parC are the most common in fluoroquinolones resistant A. baumannii isolated from Al-Azhar University hospitals
Keywords
A.baumannii; Quinolones resistance; RFLP-PCR; Molecular mechanisms; Mutations
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