EVALUATION OF PEFLOXACIN FOR TREATMENT PEKIN DUCKLINGLING SALMENLLOSIS AT SHARKIA GOVERNORATE WITH SPECIAL REFFERENCE TO ITS RESIDUCE IN TISSUES | ||||
| Mansoura Veterinary Medical Journal | ||||
| Article 1, Volume 11, Issue 2, December 2009, Page 217-229 PDF (153.86 K) | ||||
| Document Type: Original Articles | ||||
| DOI: 10.21608/mvmj.2009.158859 | ||||
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| Author | ||||
| Emam E. E | ||||
| Abstract | ||||
| A total of 120 apparently healthy, one day old Pekin duckling were used to investigate the effect of pefloxacin on Salmonella enteritidis infection. Effect of Salmonella enteritidis and pefloxacin on body weight gain and hemato-biochemical changeswas recorded as well as the residue of pefloxacin in muscles, skin and some organs were determined. Duckling were divided into four equal groups 30 for each). The 1st group apparently healthy duckling (control group), 2nd group was apparently healthy duckling and treated with pefloxacin (5mg / kgm b.wt.) in drinking water for 5days, while the duckling in 3rd & 4th groups were infected with Salmonella enteritidis at the 10th day of age. The 3rd group was kept as infected non treated duckling; Meanwhile the 4th group (infected duckling) was treated with pefloxacin (5mg kgm b.wt.) in drinking water for 5 day. Five duckling from each group were weighed individually for calculation weight gain for calculation of feed conversion rate at 1st and 21th day post treatment.At 7th and 14th day post treatment 5 duckling from each group were sacrificed for collection of 2 blood samples. The 1st sample was collected for estimation of erythrogram and the 2nd sample was collected to obtain clear serum for estimation some biochemical parameters. Another 5 duckling from each group were sacrificed samples from thigh muscle, liver, kidneys, gizzard and skin were collected at 1st,3th,7th and 10 thday post treatment for determination drug residuce Salmonella enteritidis induced 40% mortality in infected non treated pekin duckling. and 6.67% in infected group treated with pefloxacin.Healthy ducklings treated with pefloxacin revealed significant increase in the body gain, AST, ALT, alkaline phosphatase, uric acid, creatinine and significant decrease in feed conversion rate,erythrocytic count, hemoglobin content,packed cell volume, total protein and albumin. Infected ducklings with Salmonella enteritidis and non treated evoked a reduction in body weight gain, albumin and a significant increase in feed conversion rate, AST, ALT, alkaline phosphatase, uric acid, creatinin and globulin. These parameters were improved towards the normal levels when treated infected duckling with pefloxacin. Pefloxacin residue in the examined samples of liver, kidney, muscles, gizzard,skin ISSN 1110-7219 Mansoura, Vet. Med. J. (217 - 229) 218 Emam, E. E.; et al... Vol. XI, No. 2, 2009Mansoura, Vet. Med. J. INTRODUCTION Duck are a source of protein to human beings. Duck industry in Egypt has been well established; therefore any problems may be common, bacterial diseases are one of the most important problems facing duck industry (Moustafa, et al. 2008). Salmonellosis is one of the most important serious problems threatening poultry industry, where it causes serious economic losses due to high mortality (Seo, et al. 2000). Virulence factors of Salmonella include three general toxins which play role in their pathogenicity, endotoxins associated with cell wall lipopolysaccharides when liberated to circulation during bacte-rial deaths produce fever, liver and spleen lesions (Turnbull and Snoeyenbos, 1974). Antimicrobial is an important tool in reducing the losses in poultry industry. Among these antimicrobials are fluoroquinolones, which widely used in clinical practice because of their excellent antibacterial effect (Avril, et al. 1995), they act by inhibiting the activity of bacterial DNA-gyrase which responsible for supercoiling of bacterial DNA (Sun, et al. 2001). Pefloxacin is one of a 3rd generation of fluoroquinolones with broad spectrum activity against G+ve, G-ve bacteria and Mycoplasma (García, et al. 1999). Pefloxacin active against all of the Salmonella strains (Wille et al. 1988). Antibiotic residues in meat derived from treated animals and chickens could pose health threats to consumers, while the constant exposure of some microorganisms to these drugs may manifest itself in development of antibiotic resistant bacteria, allergic reactions in sensitized persons and possible direct toxic effects of minute amounts over long periods of time (Corry et al., 1983). This work was designed to evaluate the activity of pefloxacin against salmonella enteritidis infection in pekin duckling. Furthermore, effects of pefloxacin and salmonella enteritidis on some hemato-biochemical,parameter as well as drug residuce determination MATERIAL AND METHODS Drug : Pefloxacin (Peflodad 10%)® solution was obtained from Dar Al Dawa Veterinary and Agricultural Industrial Co. Itd. Jordan. Each ml contains 100mg of pefloxacin base. Experimental duckling: A total of 120 apparently healthy one day old white pekin ducklings were obtained from and fat were very high at 1st day post treatment then become very low at 5th day,Moreover the pefloxacin completely disappeared from all the examined samples after 7 thdays post treatment days The highest levels of pefloxacin residues were recorded in the liver followed by skin and fat then gizzard and muscles but the lowest levels was observed in the kidneys samples. from this study we concluded that, pefloxacin has some reversible hepatotoxicity and nephrotoxicity in healthy duckling. Salmonella enteritidis in duckling resulted in adverse effect in both erythrogram and biochemical parameters | ||||
| Keywords | ||||
| Veterinary Medicine | ||||
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