MicroRNA-146a Expression and Serum Interleukin-17 Level as potential biomarkers for Rheumatoid Arthritis | ||||
| Egyptian Journal of Chemistry | ||||
| Article 20, Volume 64, Issue 7, July 2021, Page 3423-3429 PDF (390.72 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejchem.2021.62848.3355 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Heba Ahmed Elmalt  ; Amr Mahmoud Ibrahim; Mina Wassef Girgiss
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| faculty of medicine | ||||
| Abstract | ||||
| Small non-coding RNAs are microRNAs (miRNAs) that can play a role in controlling various immune functions. MicroRNA-146a (miRNA-146a) is regarded as an essential element in posttranscriptional gene expression regulator, indicating a possible function in autoimmune diseases. Aim: The aim of the research was to evaluate the expression of miR-146a and Interleukin-17 serum levels as potential markers for rheumatoid arthritis (RA) diagnosis and to investigate its association with the activity of the disease. Methods: This research comprised 60 subjects divided into 30 RA patients and 30 healthy individuals. The rate of erythrocyte sedimentation rate (ESR), anti-cyclic-citrullinated peptide (anti-CCP) antibodies rheumatoid factor (RF), C-reactive protein (CRP) and serum IL-17 level were estimated. Using reverse transcriptase real time polymerase chain reaction quantitative, the relative quantification of miR-146a expression was determined. Results: There are highly pronounced statistical variations was observed between patients and healthy controls, with relative expression of miR-146a, (ESR), CRP, IL-17 and (anti-CC). There are also extremely important statistical differences (p < 0.001) between the various patient subgroups with respect to miR-146a relative expression. IL-17 level in the RA group was higher than in the control group. Positive associations were noticed between the levels of IL-17, ESR, CRP, (anti-CCP) and miR-146a. Conclusion: This study showed that even the expression of miR-146a was highly significant in RA patients, the level of expression was associated with the activity of the disease. Also, the increase in serum IL-17 in patients with RA compared with healthy controls played an important role in the diagnosis of the inflammatory and destructive characteristics of RA. | ||||
| Keywords | ||||
| : Rheumatoid arthritis; miR-146a; IL-17; erythrocyte sedimentation rate; C-reactive protein; anti-cyclic-citrullinated | ||||
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