Camel (Camelus Dromedarius) Milk Antibodies Ameliorated Diethylnitrosamine-Induced Hepatocellular Carcinoma in Wistar Rats
Shawki, A., El-Desouky, M., Fouad, S., Ahmed, A., Aboulhoda, B., Ahmed, W. (2021). Camel (Camelus Dromedarius) Milk Antibodies Ameliorated Diethylnitrosamine-Induced Hepatocellular Carcinoma in Wistar Rats. EKB Journal Management System, 64(8), 4611-4623. doi: 10.21608/ejchem.2021.65939.3417
Asmaa Karam Shawki; Mohamed Ali El-Desouky; Sahar Mohammed Fouad; Abd El-Fattah Mahmoud Ahmed; Basma Emad Aboulhoda; Wafaa Abdullah Ahmed. "Camel (Camelus Dromedarius) Milk Antibodies Ameliorated Diethylnitrosamine-Induced Hepatocellular Carcinoma in Wistar Rats". EKB Journal Management System, 64, 8, 2021, 4611-4623. doi: 10.21608/ejchem.2021.65939.3417
Shawki, A., El-Desouky, M., Fouad, S., Ahmed, A., Aboulhoda, B., Ahmed, W. (2021). 'Camel (Camelus Dromedarius) Milk Antibodies Ameliorated Diethylnitrosamine-Induced Hepatocellular Carcinoma in Wistar Rats', EKB Journal Management System, 64(8), pp. 4611-4623. doi: 10.21608/ejchem.2021.65939.3417
Shawki, A., El-Desouky, M., Fouad, S., Ahmed, A., Aboulhoda, B., Ahmed, W. Camel (Camelus Dromedarius) Milk Antibodies Ameliorated Diethylnitrosamine-Induced Hepatocellular Carcinoma in Wistar Rats. EKB Journal Management System, 2021; 64(8): 4611-4623. doi: 10.21608/ejchem.2021.65939.3417

Camel (Camelus Dromedarius) Milk Antibodies Ameliorated Diethylnitrosamine-Induced Hepatocellular Carcinoma in Wistar Rats

Egyptian Journal of Chemistry
Article 58, Volume 64, Issue 8, August 2021, Page 4611-4623  XML PDF (645.59 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2021.65939.3417
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Authors
Asmaa Karam Shawki email orcid 1; Mohamed Ali El-Desoukyorcid 2; Sahar Mohammed Fouad1; Abd El-Fattah Mahmoud Ahmed1; Basma Emad Aboulhodaorcid 3; Wafaa Abdullah Ahmed4
1Radioisotopes Department, Nuclear Research Center, Egyptian Atomic Energy Authority, Egypt.
2Chemistry Department, Biochemistry Division, Faculty of Science, Cairo University, Egypt
3Anatomy and Embryology Department, Faculty of medicine, Cairo University, Egypt
4Cancer Biology Department, National Cancer Institute, Cairo University, Egypt
Abstract
Hepatocellular carcinoma (HCC) is an extremely aggressive solid tumor and relates to numerous cases of cancer-associated deaths globally. Current studies reveal that natural compounds retain various therapeutic effects. This study was aimed to investigate the beneficial antitumor effects of camel (Camelus dromedarius) milk antibodies (CM-IgG) on diethylnitrosamine (DENA)-induced hepatocellular carcinoma in male Wistar rats. Hepatocarcinoma was induced in rats using DENA (50 mg/kg, twice/week) for 2 weeks followed by CCl4 (1 ml/kg, trice/week) for 6 weeks. At week 17th, HCC-bearing rats were orally administrated CM-IgG (100 mg/kg, orally) daily for 4 weeks. Liver enzyme activities and levels of alpha-fetoprotein (AFP) were measured in serum. Lipid peroxidation, nitric oxide, reduced glutathione (GSH) levels and superoxide dismutase (SOD) activity were determined in liver homogenate. Histological analysis using hematoxylin and eosin stain was examined in liver tissues. Hepatic mRNA gene expression of placental glutathione-s-transferase (GST-P) was determined by qRT-PCR. Treatment HCC-bearing rats with CM-IgG significantly reduced liver injury biomarkers and attenuated oxidative stress as well as enhanced antioxidant status. Moreover, CM-IgG significantly alleviated hepatocellular morphology alterations and down-regulated GST-P gene expression levels in liver. Our results revealed the potential role of total immunoglobulin IgG purified from camel milk in amelioration of DENA-induced hepatocellular dysfunction and oxidative stress in hepatocarcinoma-bearing rats.
Keywords
Diethylnitrosamine; Camel antibodies; HepG2 cells; Hepatocellular carcinoma; Oxidative stress; GST-P
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