Metabolomic Profiling and Antioxidant Activity of Trigonella foenum-graecum and Trigonella hamosa | ||||
Egyptian Journal of Botany | ||||
Article 82, Volume 61, Issue 2, August 2021, Page 553-564 PDF (1.91 MB) | ||||
Document Type: Regular issue (Original Article) | ||||
DOI: 10.21608/ejbo.2021.61795.1625 | ||||
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Authors | ||||
Ibrahim Abdel-Farid 1; Usama Mahalel 2, 3; Magdi El-Sayed 2, 4 | ||||
1Botany Department, Aswan Faculty of Science, Aswan University, Aswan, Egypt | ||||
2Botany Department, Faculty of Science, Aswan University, Aswan 81528, Egypt | ||||
3Biology Department, College of Science, Jouf University, Sakaka 2014, KSA | ||||
4Molecular Biotechnology Program, Field of Advanced Basic Sciences, Galala University, New Galala City, Egypt | ||||
Abstract | ||||
THE METABOLOMIC profiling of Trigonella foenum-graecum and Trigonella hamosa seeds was performed using NMR spectroscopy combined with multivariate data analysis (MVDA). The database of the chemical shifts and the J-resolved two-dimensional NMR technique were used to identify the metabolites. Additionally, the identified metabolites were confirmed by comparison with previously identified compounds as well. Among the elucidated metabolites, choline, sterols, leucine, valine, flavonoids derivatives and trigonelline were found to contribute to distinguish between the two species of Trigonella in score scatter and loading plots of multivariate data analysis. Phytochemical analysis of plant seeds extracts for total carbohydrates, proteins, phenolics, flavonoids, saponins and anthocyanins was performed by spectrophotometer. The antioxidant potential of both species was evaluated by phosphomolybdenum method and DPPH radical scavenging activity. T. foenum-graecum was characterized by higher concentrations of trigonelline, sterols, protein, flavonoids derivatives, saponins and anthocyanins, and lower IC50 in DPPH radical scavenging activity assay than T. hamosa. It was observed that total antioxidant capacity was positively correlated with glucose, trigonelline, protein, flavonoids, saponins and anthocyanins. T. foenum-graecum revealed an interesting metabolomic pattern with antioxidant activity that assigns this plant as a promising candidate to | ||||
Keywords | ||||
Antioxidant activity; Flavonoids; NMR spectroscopy; PLS-DA; Trigonelline | ||||
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