Have Methicillin Resistant Staphylococcus aureus clinical isolates to be also resistant to Streptogramins?
Attia, N., Ghazal, A., El Sherbini, E., Shalaby, M. (2021). Have Methicillin Resistant Staphylococcus aureus clinical isolates to be also resistant to Streptogramins?. EKB Journal Management System, 2(2), 286-294. doi: 10.21608/mid.2021.64988.1130
Nancy Attia; Abeer Ghazal; Eglal El Sherbini; Mona Shalaby. "Have Methicillin Resistant Staphylococcus aureus clinical isolates to be also resistant to Streptogramins?". EKB Journal Management System, 2, 2, 2021, 286-294. doi: 10.21608/mid.2021.64988.1130
Attia, N., Ghazal, A., El Sherbini, E., Shalaby, M. (2021). 'Have Methicillin Resistant Staphylococcus aureus clinical isolates to be also resistant to Streptogramins?', EKB Journal Management System, 2(2), pp. 286-294. doi: 10.21608/mid.2021.64988.1130
Attia, N., Ghazal, A., El Sherbini, E., Shalaby, M. Have Methicillin Resistant Staphylococcus aureus clinical isolates to be also resistant to Streptogramins?. EKB Journal Management System, 2021; 2(2): 286-294. doi: 10.21608/mid.2021.64988.1130

Have Methicillin Resistant Staphylococcus aureus clinical isolates to be also resistant to Streptogramins?

Microbes and Infectious Diseases
Article 14, Volume 2, Issue 2, May 2021, Page 286-294  XML PDF (457.92 K)
Document Type: Original Article
DOI: 10.21608/mid.2021.64988.1130
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Authors
Nancy Attia email orcid 1; Abeer Ghazal1; Eglal El Sherbini1; Mona Shalaby2
1Microbiology Department, Medical Research Institute, Alexandria University, Alexandria, Egypt
2Faculty of Pharmacy, Alexandria University, Alexandria, Egypt
Abstract
Background: Methicillin Resistant Staphylococcus aureus (MRSA) is a significant pathogen causing high morbidity and mortality. The extensive misuse of antibiotics has led to the willing of using older compounds like macrolide, lincosamides, and streptogramin (MLS) antimicrobials family. This study aimed to detect phenotypic and molecular characterization of macrolide, lincosamides, and streptogramins resistance in MRSA clinical isolates.
Methods: Antimicrobial susceptibility testing of 50 MRSA clinical isolates to MLS agents and quinupristin-dalfopristin (Q -D) was performed using disk diffusion method. Besides, polymerase chain reaction was conducted for amplification of genes related to streptogramins resistance (ermA, ermB, ermC, msrA, vatA, vatB, vatC, vgaA and vgbA).
Results: MLSB resistance phenotypes detected were cMLSB 9(18%), iMLSB 5(10%), MSB 8(16%), and 3(6%) isolates were LSA phenotype. No resistance to Q-D was detected in any of the tested isolates .The most prevalent MLSB resistance genes were ermC in the cMLSB and msrA in the MSB. The 3 (6%) LSA phenotype isolates expected to be SA resistant, and the 22(44%) isolates expected to be SB resistant, were sensitive to Q-D. Genes related to Q-D resistance (vatA, vatB, vatC, vgaA and vgbA genes) were not detected confirming the susceptibility of all the tested isolates to Q-D by disk diffusion method. .
Conclusion: Accurate identification of phenotypic and genotypic MLSB resistance is a crucial approach to decrease the antibiotic resistance rates. The study revealed a high prevalence of the cMLSB phenotype and the most prevalent resistance determinants was ermC.
Keywords
Methicillin Resistant Staphylococcus aureus; streptogramins; resistant genes, phenotypic; genotypic
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