SUSCEPTIBILITY OF DESERT LOCUST, SCHISTOCERCA GREGARIA (ORTHOPTERA: ACRIDIDAE) TO BACILLUS CEREUS ISOLATED FROM EGYPT
Reda, M., Mashtoly, T., El-Zemaity, M., Abolmaaty, A., Abdelatef, G., Marzouk, A. (2018). SUSCEPTIBILITY OF DESERT LOCUST, SCHISTOCERCA GREGARIA (ORTHOPTERA: ACRIDIDAE) TO BACILLUS CEREUS ISOLATED FROM EGYPT. EKB Journal Management System, 26(2), 725-734. doi: 10.21608/ajs.2018.16005
M. Reda; T. A. Mashtoly; M. S. El-Zemaity; A. Abolmaaty; G. M. Abdelatef; A. A. Marzouk. "SUSCEPTIBILITY OF DESERT LOCUST, SCHISTOCERCA GREGARIA (ORTHOPTERA: ACRIDIDAE) TO BACILLUS CEREUS ISOLATED FROM EGYPT". EKB Journal Management System, 26, 2, 2018, 725-734. doi: 10.21608/ajs.2018.16005
Reda, M., Mashtoly, T., El-Zemaity, M., Abolmaaty, A., Abdelatef, G., Marzouk, A. (2018). 'SUSCEPTIBILITY OF DESERT LOCUST, SCHISTOCERCA GREGARIA (ORTHOPTERA: ACRIDIDAE) TO BACILLUS CEREUS ISOLATED FROM EGYPT', EKB Journal Management System, 26(2), pp. 725-734. doi: 10.21608/ajs.2018.16005
Reda, M., Mashtoly, T., El-Zemaity, M., Abolmaaty, A., Abdelatef, G., Marzouk, A. SUSCEPTIBILITY OF DESERT LOCUST, SCHISTOCERCA GREGARIA (ORTHOPTERA: ACRIDIDAE) TO BACILLUS CEREUS ISOLATED FROM EGYPT. EKB Journal Management System, 2018; 26(2): 725-734. doi: 10.21608/ajs.2018.16005

SUSCEPTIBILITY OF DESERT LOCUST, SCHISTOCERCA GREGARIA (ORTHOPTERA: ACRIDIDAE) TO BACILLUS CEREUS ISOLATED FROM EGYPT

Arab Universities Journal of Agricultural Sciences
Article 26, Volume 26, Issue 2 - Serial Number 1, September 2018, Page 725-734  XML PDF (391.28 K)
Document Type: Original Article
DOI: 10.21608/ajs.2018.16005
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Authors
M. Reda* 1; T. A. Mashtoly2; M. S. El-Zemaity2; A. Abolmaaty3; G. M. Abdelatef4; A. A. Marzouk5
1Locust and Grasshoppers Research Dept., Plant Protection Research Institute, Agric.
2Plant Protection Dept., Fac. of Agric., Ain Shams Univ., Cairo, Egypt.
3Food Sci. Dept., Fac. of Agric., Ain Shams Univ., Cairo, Egypt.
4Locust and Grasshoppers Research Dept., Plant Protection Research Institute, Agric. Research Centre. Dokki, Giza, Egypt
5Microbiological Resources Centre (MIRCEN), Fac. of Agric., Ain Shams Univ., Cairo, Egypt
Abstract
Examination was done at preliminary bracketing bioassay on one old 4th nymphal instar of desert locust. Results showed that two isolates, namely NDL1 and NDL2 were having highly potentiality as entomopathogenic bioagents. Thirty isolates were isolated from dead/ infected nymphs of desert locust occurred in raring cages at Department of Locust and Grasshoppers Research, Plant Protection Research Institute, Agricultural Research Center, Dokki, Giza, Egypt. Molecular identification of isolated bacteria was done using universal primers of 16s rRNA, followed by DNA sequencing. Nucleotides were blasted at (https://www.ncbi. nlm.nih.gov /genbank/) to recognize that NDL1 and NDL2 isolates were two different isolates of Bacillus cereus with a high similarity (100%).  Susceptibility of 4th nymphal instar of Schistocerca gregaria (Forskal) to the isolated B. cereus was determined using two bioassay procedures, Leaf-dip and per os. The insecticidal activity of both isolates against locust nymph in leaf dipping showed that NDL2 was more efficient than NDL1. However, the opposite trend was observed in using per os.  Both Isolates have the potential to be a successful biocidal agent to control desert locust.
Keywords
Schistocerca gregaria; biological control; entomopathogenic bacteria; 16S rRNA genes
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