Preparation and Clinical Use of Autologous Platelet Rich Plasma Eye Drops in Dogs
ahmed, E., Misk, T., Gadallah, S., Ghaith, A. (2021). Preparation and Clinical Use of Autologous Platelet Rich Plasma Eye Drops in Dogs. EKB Journal Management System, 3(1), 100-105. doi: 10.21608/jcvr.2021.160259
eslam reda ahmed; Tarek Misk; Shaaban Gadallah; Alaa Ghaith. "Preparation and Clinical Use of Autologous Platelet Rich Plasma Eye Drops in Dogs". EKB Journal Management System, 3, 1, 2021, 100-105. doi: 10.21608/jcvr.2021.160259
ahmed, E., Misk, T., Gadallah, S., Ghaith, A. (2021). 'Preparation and Clinical Use of Autologous Platelet Rich Plasma Eye Drops in Dogs', EKB Journal Management System, 3(1), pp. 100-105. doi: 10.21608/jcvr.2021.160259
ahmed, E., Misk, T., Gadallah, S., Ghaith, A. Preparation and Clinical Use of Autologous Platelet Rich Plasma Eye Drops in Dogs. EKB Journal Management System, 2021; 3(1): 100-105. doi: 10.21608/jcvr.2021.160259

Preparation and Clinical Use of Autologous Platelet Rich Plasma Eye Drops in Dogs

Journal of Current Veterinary Research
Article 12, Volume 3, Issue 1, April 2021, Page 100-105  XML PDF (267.47 K)
Document Type: Original Article
DOI: 10.21608/jcvr.2021.160259
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Authors
eslam reda ahmed email 1; Tarek Misk2; Shaaban Gadallah2; Alaa Ghaith3
1faculty of veterinary medicine Sadat city university, egypt
2Department of Surgery, Faculty of Vet. Medicine, University of Sadat City, Egypt.
3Department of Ophthalmology, Faculty of Medicine, University of Alexandria, Egypt.
Abstract
Several studies demonstrate the effectiveness of eye drops Platelet Rich Plasma (E-PRP) as a promoter of the healing of different ophthalmological disorders in human, due to the high concentration of growth factors as EGF, PDGF-BB and Fibronectin which represent the key to obtain the desired regenerative effects on the ocular surface. There is different protocol for PRP eye drops preparation for use in human corneal disorders. This study was to evaluate an easy and affordable technique for preparation of canine autologous E-PRP according to standard measure used before for human corneal disorders to be used in canine corneal ulcers in comparison with control group, all treatments were applied four times daily. Twelve healthy dogs were divided into two groups first group included eight dogs which received autologous E-PRP compared with second group which included four dogs and received saline eye drops as control. Complete blood count is performed to whole blood and E-PRP to validate last one content of platelets which must equal more 1.5 those in whole blood. E-PRP contain platelets 2.41-fold than those in whole blood with one step centrifugation technique. Autologous canine E-PRP mean healing time was 3.62±0.23 days while with control group mean healing time was 6.75±0.30 days. In conclusion, As the E-PRP is now widely documented in human ophthalmology, it had been proven as a great result in veterinary practice when prepare autologous E-PRP by single step centrifugation to be used for canine corneal wound and healing rate was better than control group by significant difference. which make it novel option for treatment for canine corneal ulcer.

Keywords
E-PRP; corneal ulcer; corneal wound healing; dogs
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