Intermediate and pharmaceutical compounds associated with fermentation of spoilage date fruits by Hanseniaspora guilliermondii KKUY-0045 | ||||
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology | ||||
Article 1, Volume 6, Issue 1, June 2014, Page 1-12 PDF (399.06 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/eajbsc.2014.16042 | ||||
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Authors | ||||
Mohamed Hashem1; Abd El-Latif Hesham2; Saad A. Alamri3; Sulaiman A. Alrumman3 | ||||
1- Faculty of Science, Botany and Microbiology Department, Assiut University, Assiut, Egypt - Faculty of Science, Biology Department, King Khalid University, Abha, Saudi Arabia | ||||
2-Faculty of Science, Biology Department, King Khalid University, Abha, Saudi Arabia - Faculty of Agriculture, Genetics Department, Assiut University, Assiut, Egypt | ||||
3Faculty of Science, Biology Department, King Khalid University, Abha, Saudi Arabia | ||||
Abstract | ||||
The aim of this study was to determine the intermediate chemical compounds during the fermentation of spoilage date juice (SDJ) by newly isolated yeast strain Hanseniaspora guilliermondii KKUY-0045. This yeast strain was identified based on sequence of the variable D1/D2 domain of the large subunit (26S) ribosomal DNA and comparing it with similar sequences in the Genbank database. Phylogenetic analysis using rDNA sequences indicated that strain KKUY-00045 shared one cluster with Hanseniaspora guilliermondii. In 7-L fermenetor, this strain produced 87.74 g/L of ethanol after 3 days of incubation. The chemical analysis of the cell free extract of the yeast strain grown on the SDJ by using GC-MS revealed the presence of some important chemical compound such as pregnane and nonacosane that could be used in the medical and pharmaceutical industries. The study recommends extensive studies to discover the chemicals associated with fermentation process and their industrial role. | ||||
Keywords | ||||
Fermentation Hanseniaspora guilliermondii; GC-MS; Pregnane and nonacosane; 26S rDNA sequencing; Phylogenetic analysis | ||||
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