Molecular characterization of Paenibacillus larvae larvae, for early diagnosis of american foulbrood of honeybees in Egypt
Ma’moun, S., Imam, M., Nafea, E., Anan, R., Salama, M., El Shafai, A., Kaschef, A. (2014). Molecular characterization of Paenibacillus larvae larvae, for early diagnosis of american foulbrood of honeybees in Egypt. EKB Journal Management System, 6(1), 35-45. doi: 10.21608/eajbsc.2014.16045
Shireen A. M. Ma’moun; Mohamed I. Imam; Emad A. Nafea; Rabia A. Anan; Mohamed S. Salama; Akila M. El Shafai; Ahmed H. Kaschef. "Molecular characterization of Paenibacillus larvae larvae, for early diagnosis of american foulbrood of honeybees in Egypt". EKB Journal Management System, 6, 1, 2014, 35-45. doi: 10.21608/eajbsc.2014.16045
Ma’moun, S., Imam, M., Nafea, E., Anan, R., Salama, M., El Shafai, A., Kaschef, A. (2014). 'Molecular characterization of Paenibacillus larvae larvae, for early diagnosis of american foulbrood of honeybees in Egypt', EKB Journal Management System, 6(1), pp. 35-45. doi: 10.21608/eajbsc.2014.16045
Ma’moun, S., Imam, M., Nafea, E., Anan, R., Salama, M., El Shafai, A., Kaschef, A. Molecular characterization of Paenibacillus larvae larvae, for early diagnosis of american foulbrood of honeybees in Egypt. EKB Journal Management System, 2014; 6(1): 35-45. doi: 10.21608/eajbsc.2014.16045

Molecular characterization of Paenibacillus larvae larvae, for early diagnosis of american foulbrood of honeybees in Egypt

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
Article 4, Volume 6, Issue 1, June 2014, Page 35-45  XML PDF (402.9 K)
Document Type: Original Article
DOI: 10.21608/eajbsc.2014.16045
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Authors
Shireen A. M. Ma’moun1; Mohamed I. Imam1; Emad A. Nafea2; Rabia A. Anan1; Mohamed S. Salama1; Akila M. El Shafai1; Ahmed H. Kaschef1
1Department of Entomology, Faculty of Science, Ain Shams University, Cairo, Egypt
2Honeybees' Research Center, Plant Protection Institute, Cairo, Egypt
Abstract
A reliable procedure for early detection of Paenibacillus larvae subsp. larvae (P. l. larvae), the causal agent of American Foulbrood disease (AFB) of honeybees (Apis mellifera L.) based on the polymerase chain reaction (PCR) and subspecies – specific KAT primers. A PCR amplicon of the expected size 550 bp only found in P. l. larvae strains was used for positive AFB. This PCR assay provides a specific detection for P. l. larvae from week 1 post infection even if there is no clinical symptoms appeared in a colony.
The technique can be directly used to detect presence or absence of P. l. larvae spores in honeybee samples and contaminated honeys.
Keywords
Molecular-; Paenibacillus larvae; Honeybees; American foulbrood; Egypt
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