Effect of Benzoic and Sorbic Acids on Mycotoxins Inhibition in Edible Broad Bean Seeds

Youssef, Nesreen; El samra, Ibrahim; Abdelbary, Ahmed Ibrahim

doi:10.21608/jalexu.2014.160517

	Effect of Benzoic and Sorbic Acids on Mycotoxins Inhibition in Edible Broad Bean Seeds
Journal of the Advances in Agricultural Researches
Article 6, Volume 19, Issue 3, September 2014, Page 482-497 PDF (769.12 K)
Document Type: Research papers
DOI: 10.21608/jalexu.2014.160517
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Authors
Nesreen Youssef¹; Ibrahim El samra^* ²; Ahmed Ibrahim Abdelbary²
¹Regional Centre for Food and Feed, Alexandria, Agricultural Researches Centre , Egyptian Ministry of Agriculture, El -Giza, Cairo, Egypt
²Department of Agricultural Botany, Faculty of Agriculture(Saba-Bacha), Alexandria University, P. O. Box 21531, Bolkley, Alexandria, Egypt.
Abstract
Natural infected broad bean seeds were collected from Noubaria region during the season of 2012-2013. The isolated and purified fungi from these seeds were identified as: Alternaria alternata, Penicilliun citrinum and Aspergillus flavus. Occurrencefrequencies of the isolated fungi were 75.76, 15.16 and 9.08%, respectively. Toxin production potentials of Alternaria alternata and P. citrinum isolates, as estimated by Plug Agar method, were 231.00 and 337.00 µ.g/g, respectively., whereas, A.flavus isolate was unable to produce aflatoxins. Sorbic and benzoic acids were proved to be more effective in reducing fungal growth of A. alternata and P. citrinum than metalaxyl and ridomyl fungicides. A. alternata was less sensitive to sorbic acid than benzoic acid. All the tested treatments, at their MICs, significantly reduced growth of A. alternata growth and alternariol (AOH) production; however, higher efficiency ratio (99.860%) was realized by benzoic acid. Similar inhibition of growth and citrinin (CTN) production was induced by P. citrinum. Metalaxyl was less efficient in inhibiting AOH and CTN than the other tested treatments. Soaking seeds in water significantly raise the efficiency of mycotoxins production in all the tested seed treatments. Moreover, efficiency of benzoic and sorbic acids in reducing AOH and CTN production was higher during soaking process. Seeds inoculated with the tested pathogens, then treated with sorbic or benzoic acid appeared healthy with normal color, texture and morphology.
Keywords
Mycotoxins inhibition; broadbean seed diseases; Alternaria alternate; Penicillium citrinum; Benzoic acid; Sorbic acid; metalaxyl; Ridomyl


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Effect of Benzoic and Sorbic Acids on Mycotoxins Inhibition in Edible Broad Bean Seeds

Elizabeth, M. H. 2008. Exploration of structure and function in biomolecules through solid-state NMR and computational methods. Book, google.com.eg. C.F., phD in the subject of physics. Pp. 119-125. Utah University., U.S.A.

Embaby, E. S. M., M. Hazaa, L. F. Hagag, T. E. S. Ibrahim and F. S. Abd El-Azem. 2013. Decay of some citrus fruit quality caused by fungi and their control: II- Control of Alternaria rot or core rot decay by using some alternative fungicides. Journal of Applied Sciences Research, 9(11): 5671-5678.

Enyiukwu, D. N., A. N. Awurum and J. A. Nwaneri. 2014. Mycotoxins in stored agricultural products: Implications to food safety and health and prospects of plant-derived pesticides as novel approach to their management. Greener Journal of Microbiology and Antimicrobials, 2(3): 32- 48.

Erich, L. 1980. Sorbates in antimocrobial food additives: characteristics, uses, effects. Translated from German by Grant F. Edwards, Springer-Verlag, Berlin, Heidelberg, New York. pp 257.

Kawamura, C., T. Tsujimoto and T. Tsuge. 1999. Targeted disruption of a melanin biosynthesis gene affects conidial development and UV tolerance in the Japanese pear pathotype of Alternaria alternata. Mol. Plant-Microbe Interact., 12: 59-63. [PubMed].