Phytochemical analysis and inhibitory effects of extract of young fruits of Ficus palmate on some pathogenic microbes | ||||
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology | ||||
Article 13, Volume 6, Issue 1, June 2014, Page 131-139 PDF (366.48 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/eajbsc.2014.16054 | ||||
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Authors | ||||
Sulaiman A. Alrumman1; Mahmoud F. Moustafa2; Abd El-Latif Hesham3; Saad A. Alamri1; Mohamed Hashem4 | ||||
1Department of Biology, Faculty of Science, King Khalid University, Abha, Saudi Arabia | ||||
2Department of Botany, Faculty of Science, South Valley University, Qena, Egypt | ||||
3Genetics Department, Faculty of Agriculture, Assiut University, Assiut, Egypt | ||||
4Faculty of Science, Botany and Microbiology Department, Assiut University, Assiut, Egypt | ||||
Abstract | ||||
The present study was conducted to determine the antimicrobial activity of various extracts of young fruits of Ficus palmata Forsk. against some Gram positive and Gram negative bacteria and Candida species using the agar well diffusion method. Results showed that the cold water extract had no inhibitory effect against any tested microbes, while the hot water extract inhibited the microbial growth (12.5%). Methanol, chloroform, petroleum ether or acetone extract of the dry fruits inhibited microbial growth (37.5%), while diethyl ether extract inhibition was 12.5%. Methanol extract of fresh fruitsinhibited the microbial up to 75%. However, the chloroform and petroleum ether extracts inhibited microbial growth by 62.5% and 37.5%, respectively. Gas Chromatogram- Mass spectrometry (GC-MS) analysis of hexan extract of the young fruits of Ficus palmata was carried out to identify its phytochemical constituents. Four chemical constituents have been identified: diethyl phthalate was the major compound (86.19%) (Retention time 16.65), and cyclohexasiloxane, dodecamethyl-, (Rt 12.77), 1,3-dimethyl-2-nitrobenzene (Rt 14.91) and benzamide (Rt 15.07) were the minor constituents (4.47, 7.96 and 1.36%, respectively). It could be concluded that the extract of young fresh fruits of Ficus palmata and its bioactive components, in particular diethyl phthalate, could be of phytopharmaceutical importance as an antimicrobial agents | ||||
Keywords | ||||
Ficus palmata; Gram positive bacteria; Gram negative bacteria; Candida species | ||||
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