Biological and molecular effects of pyriproxyfen, insect growth regulator (IGR), on Met gene in susceptible wild type and Met mutant Drosophila | ||||
| Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology | ||||
| Article 2, Volume 5, Issue 2, December 2013, Page 11-18 PDF (750.62 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/eajbsc.2013.16091 | ||||
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| Authors | ||||
| Reda F. A. Bakr; Jehane A. Hafez; Thorya F. K. Alnagar; Dalia A. M. Salem; Ola H. Zyaan | ||||
| Department of Entomology, Faculty of Science, Ain Shams Univ., Abbassia- Cairo, Egypt | ||||
| Abstract | ||||
| Juvenile hormone (JH) signaling pathway at the molecular level is still not well understood. Methoprene-tolerant (Met) in Drosophila melanogaster fulfills many of the requirements for a hormone receptor gene. However, Met mutant flies are resistant to insect growth regulators (IGRs). In our study, the resistance to juvenile hormone agonist (JHA) in Methoprene-tolerant (Met) mutant flies was studied. Met mutant and susceptible wild type larvae were treated by different concentrations of pyriproxyfen at 0, 0.03, 0.1, 0.3, 1, and 3 ppm. We observed that the mean percentage mortalities in Met mutant were significantly (P<0.05) lower than that in the susceptible wild type. Also, resistance in insects to IGRs due to Met gene was studied using PCR and DNA sequencing analyses. We found that there are no changes in the DNA sequences of Met gene after the treatment with pyriproxyfen. | ||||
| Keywords | ||||
| pyriproxyfen- Met gene- Drosophila melanogaster | ||||
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