Molecular genetic identification of two bracon species based on RAPD-PCR and 16S rRNA genes
Bakr, R., Gesraha, M., Guneidy, N., Farag, N., Ebeid, A., Elbehery, H., Abou-Ellail, M. (2013). Molecular genetic identification of two bracon species based on RAPD-PCR and 16S rRNA genes. EKB Journal Management System, 5(2), 99-107. doi: 10.21608/eajbsc.2013.16099
Reda F.A. Bakr; Mohamed Ahmed Gesraha; Noha Awny Mohamed Guneidy; Nagy Abd Elatief Farag; Amany Ramadan Ebeid; Hoda Hassan Ali Elbehery; Mohamed Abou-Ellail. "Molecular genetic identification of two bracon species based on RAPD-PCR and 16S rRNA genes". EKB Journal Management System, 5, 2, 2013, 99-107. doi: 10.21608/eajbsc.2013.16099
Bakr, R., Gesraha, M., Guneidy, N., Farag, N., Ebeid, A., Elbehery, H., Abou-Ellail, M. (2013). 'Molecular genetic identification of two bracon species based on RAPD-PCR and 16S rRNA genes', EKB Journal Management System, 5(2), pp. 99-107. doi: 10.21608/eajbsc.2013.16099
Bakr, R., Gesraha, M., Guneidy, N., Farag, N., Ebeid, A., Elbehery, H., Abou-Ellail, M. Molecular genetic identification of two bracon species based on RAPD-PCR and 16S rRNA genes. EKB Journal Management System, 2013; 5(2): 99-107. doi: 10.21608/eajbsc.2013.16099

Molecular genetic identification of two bracon species based on RAPD-PCR and 16S rRNA genes

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
Article 10, Volume 5, Issue 2, December 2013, Page 99-107  XML PDF (1014.08 K)
Document Type: Original Article
DOI: 10.21608/eajbsc.2013.16099
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Authors
Reda F.A. Bakr1; Mohamed Ahmed Gesraha2; Noha Awny Mohamed Guneidy3; Nagy Abd Elatief Farag4; Amany Ramadan Ebeid4; Hoda Hassan Ali Elbehery4; Mohamed Abou-Ellail5
1Department of Entomology,Faculty of Science Ain Shams University Cairo, Egypt
2Pests and Plant Protection Dept., Agricultural and Biological Division, National Research Center, Dokki, Cairo, Egypt.
3Department of Entomology,Faculty of Science Ain Shams University Cairo, Egypt.
4Pests and Plant Protection Dept., Agricultural and Biological Division, National Research Center, Dokki, Cairo, Egypt
5Department of Cytology and Genetics, Genetic Engineering and Biotechnology Division, National Research Centre, Cairo, Egypt
Abstract
Random amplified polymorphic DNA (RAPD)-PCR genomic fingerprinting and partial sequencing of the 16S rRNA gene were evaluated on two insects collected from the Egyptian field which could belong to B. hebetor and B. brevicornis to investigate their genetic relatedness and to establish the value of techniques for their identification. Nearly identical RAPD-PCR profiles and identical 467 bp fragments of the 16S rRNA genes indicated many of genetic diversity between the two insects under study. The low levels of similarity (78.21% in the partial 16S rRNA genes and 86% in RAPD-PCR) appeared between the insects B. hebetor egypt and B. brevicornis egypt. However, 16S rRNA genes and RAPD-PCR provided an effective means of differentiating between members of the taxa. Moreover, a phylogenetic tree constructed from 16S rDNA sequences showed that B. hebetor egypt clustered with the B. hebetor with a degree of similarity 92%, but B. brevicornis Egypt clustered in a separated group. However, RAPD-PCR and partial sequencing of the 16S rDNA analysis raises questions about the taxonomic positioning of the two insects isolated from the Egyptian environment.
Keywords
RAPD-PCR and 16S rRNA genes; bracon species
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