Ribosomal DNA sequences reveal a parasitic septate gregarine (Apicomplexa: Gregarinia) in the scorpion Leiurus quinquestriatus in Egypt | ||||
| Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology | ||||
| Article 15, Volume 5, Issue 1, June 2013, Page 123-129 PDF (140.93 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/eajbsc.2013.16118 | ||||
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| Author | ||||
| Moustafa M. H. Sarhan | ||||
| Department of Zoology, Faculty of Science, Al-Azhar University, Assiut | ||||
| Abstract | ||||
| Ribosomal RNA genes are ubiquitously present and nucleotide sequences of many regions are relatively conserved among many organisms. Many specific PCR primers have been developed for amplification of the rDNA fragments from particular taxa. However, many authors use universal primers which can bind to conserved regions to detect protozoa pathogens that are obligate parasites of digestive tracts and body cavities in the analysed DNA sample of invertebrate animals. In this study, uncultured gregarine clones were detected by PCR and rDNA sequencing in the scorpion, Leiurus quinquestriatus (Scorpiones: Buthidae). Comparison of the amplified rDNA fragments with sequences deposited in GenBank revealed that analysed sequences corresponded to 18S rDNA from the genus Gregarina (88 % identity with Xiphocephalus ellisi). To our knowledge, this is the first record of scorpion infected with gregarines. | ||||
| Keywords | ||||
| rDNA; Leiurus quinquestriatus; Scorpion; Apicomplexa; gregarines | ||||
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