Effects of Supplementing Extenders with L-Arginine on Semen Cryopreservation and Fertility in Buffalo Bulls | ||||
Journal of Animal and Poultry Production | ||||
Article 14, Volume 11, Issue 12, December 2020, Page 591-595 PDF (646.28 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/jappmu.2020.161209 | ||||
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Authors | ||||
Entesar Z. Eliraqy 1; E. A. Abd-Allah1; Mogeda M. A. Salem2; W. F. M. Fouad3 | ||||
1Biotechnology Res. Dep. Animal Production Res. Institute, Agric. Res.Center, Dokki, Giza, Egypt. | ||||
2Animal Production Res. Institute, Agric. Res.Center, Dokki, Giza, Egypt. | ||||
3Cattle Breed Res. Dep. Animal Production Res. Institute, Agric. Res.Center, Dokki, Giza, Egypt. | ||||
Abstract | ||||
Knowledge of influence added more one concentration of L-arginine to enhance the poor quality of buffalo bull spermatozoa considers the main objective in our study. Semen collected from five buffalo bulls once weekly and ejaculates with 45-50% mass motility were pooled. Semen was diluted at 37C° by Tris-egg yolk extender, also separated to five portions, 1st portion without supplement it consider control to other portions and 2nd, 3rd, 4th and 5th were supplemented with 3, 5, 7 and 10 mM L-arginine respectively. Progressive sperm motility, live sperm%, abnormal sperm%, chromatin normality, Conception rate, and integrity of sperm acrosome were determined. Data declared the, extenders with concentrations of 7 and 10 mM L-Arginine was improved all parameters studied than 3 and 5 mM L-arginine extenders and control extender at different cryopreservation processes. A significant difference in chromatin integrity was detected at all treatments. Also, the conception rate was higher in buffalo cows artificially inseminated with 7 and 10 mM L-arginine (67.27 and 71.70 %) compared with control (45.45%). Conclusively, it could be concluded that adding L-arginine with concentrations of 7 or 10mM to Tris-extender improved the cryopreservation and fertility of the poor quality of Buffalo bull spermatozoa. | ||||
Keywords | ||||
L- Arginine; Semen extender; Semen quality; Chromatin integrity; Buffalo bull | ||||
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