Changes Of P21 And Bcl-2 During Induction Of Bladder Inflammation And/Or Bladder Carcinogenesis | ||||
The Egyptian Journal of Hospital Medicine | ||||
Article 20, Volume 49, Issue 1, October 2012, Page 781-792 PDF (277.88 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/ejhm.2012.16214 | ||||
View on SCiNiTO | ||||
Authors | ||||
A.A. Sayed1; M.A. El-Desoky2; S. Shaarawy3; A.M. Ashmawy3 | ||||
1M.Sc candidate, Faculty of Science, Cairo University | ||||
2Department of Chemistry,BiochemistryDivision, Faculty of Science, Cairo University | ||||
3Department of Medical Biochemistry,NCI , Cairo University | ||||
Abstract | ||||
The effect of E.coli infection in association with dibutylamine and sodium nitrate in induction of bladder inflammation and /or bladder carcinogenesis were investigated in 150 male albino rats, divided into five groups, as follows: The first group(G1) infected by E. coli , The second group (G2), given nitrosamine precursors in the diet , The third group(G3) infected by E. coli and given nitrosamine precursors in the diet ,The fourth group(G4) infected by E. coli , given nitrosamine precursors in the diet and received standard diet containing 1% Curcumin powder mixed in the diet and the fifth group (G5) , served as control group . Results: The worst histopathological changes are in G3 . A highly significant decrease in the mean cell lysate level of p21was found in different studied groups(G1,G2&G3) especially in(G3) where P values in these groups when compared to control group were (P < 0.0001) . In curcumin treated group(G4), there were downregulation in the mean cell lysate level of p21 gene when compared to control group but higher than other studied groups (G1,G2&G3) . A highly significant increase in the mean serum level of Bcl-2 was found in different studied groups(G1,G2&G3) especially in(G3) where P values in these groups (G1,G2,G3) when compared to control group were (P < 0.0001). (G4) showed higher Bcl-2 serum level when compared to control group, this difference was insignificant but this level is lower than other studied groups (G1,G2&G3) . Conclusion: The results in the present study indicate that both P21 and Bcl-2 can be used as biological markers in the diagnosis of bladder cancer . Curcumin have the ability to overcome the decrease in p21WAF1/CIP1 protein and the increase of Bcl-2 protein and reduce the induction of carcinogenic effect . | ||||
Keywords | ||||
Bladder cancer; Bladder inflammation; P21; Bcl-2 and Curcumin | ||||
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