Micropropagation and Genetic Variations of Cordyline terminalis cv. Red Top | ||||
| Journal of the Advances in Agricultural Researches | ||||
| Article 13, Volume 23, Issue 2 - Serial Number 87, June 2018, Page 328-349 PDF (2.28 MB) | ||||
| Document Type: Research papers | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Mohmed Gamal Eltorky1; Mohmed Elsaid Elmahrouk2; Hoda Elmokadem1; Heba El-Sammak1 | ||||
| 1Dept.of Floriculture, Ornamental Horticulture and Landscape Gardening, Faculty of Agriculture, Alexandria University. | ||||
| 2Horticulture Department, Faculty of Agriculture, Kafrelsheikh University | ||||
| Abstract | ||||
| Due to the slow, expensive and time - consumed conventional propagation of Cordyline , and the merits of in vitro propagation for overcoming on these problems. The present study was undertaken. Simple protocol for in vitro regeneration of Cordyline terminalis cv. Red Top using shoot tip explants was achieved. For initiation stage , shoot tip explants were cultured on MS medium fortified with both 3% sucrose (w/v) and 0.2% gelrite (w/v). As for multiplication stage, the new formed axillary shoots were cultured on MS fortified with various concentrations of benzyladenine (BA; 0,1, 3, 5 and 7 mg/l) and thidiazuron (TDZ; 0, 0.5 ,1, 1.5 and 2 mg/l) in the presence of 1 mg/l Kinetin (Kin) were used. As for shoot elongation and in vitro rooting stage, new formed shoots derived from multiplication stage were culture on MS- PGRs -free medium gelled with 2% (w/v)gelrite . Concerning the acclimatization stage, the rhizogened plantlets were potted in mixture media consists of peat moss and vermiculite (1:1 %). The survival percentage of new formed plantlets after acclimatization was 100 % of the treatments; 1 mg/l BAP +1 mg/l Kin, 7 mg/l BAP +1 mg/l Kin and 1.5 mg/l TDZ +1 mg/l Kin under the light intensity 30 μmol m-2 s-1. The procedure outlined has fulfilled the requirements for micropropagation of Cordyline. Randomly amplified polymorphic DNA (RAPD) analysis showed that the polymorphism level was the same at all primers (100%). | ||||
| Keywords | ||||
| cordyline terminalis; Micropropagation; regeneration; RAPD; somaclonal variation | ||||
| References | ||||
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