EFFECT OF STRESS CONDITIONS ON STABILITY AND BIOLOGICAL ACTIVITY OF A RECOMBINANT Escherichia Coli AGAINST THE COTTON LEAFWORM SPODOPTERA LITTORALIS (BOISD)

GAMAL, RAWIA F.; AL-FATEH, SAMER R.; MOHAMMAD, ABEER M.; EL- SAWY, MOHAMED

doi:10.21608/ejar.2013.163072

	EFFECT OF STRESS CONDITIONS ON STABILITY AND BIOLOGICAL ACTIVITY OF A RECOMBINANT Escherichia Coli AGAINST THE COTTON LEAFWORM SPODOPTERA LITTORALIS (BOISD)
Egyptian Journal of Agricultural Research
Article 3, Volume 91, Issue 2, July 2013, Page 413-427 PDF (367.01 K)
Document Type: Original Article
DOI: 10.21608/ejar.2013.163072
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Authors
RAWIA F. GAMAL¹; SAMER R. AL-FATEH²; ABEER M. MOHAMMAD²; MOHAMED EL- SAWY¹
¹Dept. of Microbiology, Fac. of Agriculture, Ain Shams Univ.
²Plant Protection Research Institute, ARC, Dokki, Giza, Egypt
Abstract
The effect of stress conditions on viability and activity of the recombinant E. coli, that contains cryIAC gene, which encodes a crystal toxin specific to Spodoptera littoralis, was tested. Solar radiation showed the worst effect on both viability and activity against the larvae of S. littoralis. The drastic effect also happened when recombinant E. coli was exposed to heat treatment at 50oC for more than 5 min. and to high concentrations of sucrose (> 20 % for more than 2 days). Also, the same effect happened when cells were exposed to NaCl > 5 %, even after one day of exposure. The viability of E. coli cells after preservation by freezing under glycerol, lyophilization and encapsulation indicated that, freezing was the best method for keeping both viability and activity of cells till 9 months post preparation.
Keywords
ACTIVITY OF A RECOMBINANT; SPODOPTERA LITTORALIS (BOISD


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