Inducing some Morphological Variations in Cyperus alternifolius L. by Using Gamma Irradiation | ||||
| Journal of the Advances in Agricultural Researches | ||||
| Article 5, Volume 24, Issue 2 - Serial Number 92, June 2019, Page 178-193 PDF (1.7 MB) | ||||
| Document Type: Research papers | ||||
| DOI: 10.21608/jalexu.2019.163073 | ||||
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| Author | ||||
| Magd el Din Rida | ||||
| Antoniades Research Branch Alexandria, Ornamental Plants Research and Landscape Gardening Department, Horticulture Research Institute, ARC, Egypt. | ||||
| Abstract | ||||
| This study was conducted during 2015, 2016 and 2017 seasons at Antoniades Research Branch, Horticulture Research Institute, Ministry of Agriculture, Alexandria. The aim of the present study was to study the effects of different doses of gamma rays from Cobalt -60 on Cyperus alternifolius L. plants Rhizomes of Cyprus plants were irradiated with six doses of gamma irradiation (0.0,20, 40, 60, 80 and 100 Gray). The results showed that irradiation of rhizomes with 20 Gray caused the highest increase in sprouting percentage and rate while the 100 gray treatment caused the lowest survival percentage in M1 –generation. The 100 Gray treatment caused the lowest significant decrease in all studied vegetative growth parameters in M1 and M2 generations. As for the vegetative growth abnormalities, the application of gamma rays at the dose of 100 Gray resulted in dwarf plants in M1 and M2 generations. While using 40 Gray caused 3 regenerated small plants from leaves internode and 60 Gray caused 1 regenerated small plant from leaves internodes in M1 –generation , this abnormality disappeared in the M2 –generation. Application of restriction fragment length polymorphism (RFLP) analysis on three Cyperus alternifolius generations stressed with 60 and 100 Gray comparing with un-irradiated Cyperus alternifolius cleared that radiation dose (100 Gray) reflected the dramatic increase of genetic polymorphism and first generation reflected the highest radiation influence on polymorphism. | ||||
| Keywords | ||||
| Gamma rays - Cyperus alternifolius L - Vegetative growth abnormalities; Dwarf plants - RFLP analysis | ||||
| References | ||||
|
Abdel-Hady, M.S., E.M. Okasha, S.S.A. Soliman and M. Talaat (2008). Effect of Gamma radiation and gibberellic acid on germination and alkaloid production in Atropa belladonna. Aust. J.Basic Appl., 2(3): 401-405.
Anupam T, Singh A. K. and P. Sumit (2018). Effect of gamma irradiation on growth and floral characters of gladiolus varieties. Int. J. of Chemical Studies, 6(6): 1277-1282
Brasch J, V. Beck-Jendroschek and R. Gläser (2010). Fast and sensitive detection of Trichophyton rubrum in superficial tinea and onychomycosis by use of a direct polymerase chain reaction. Blackwell Verlag Gmb H. :54(5):e313-7.
Danylchenko O.and B. Sorochinsky (2005). Use of RAPD assay for the detection of mutation changes in plant DNA induced by UV-B and Rrays BMC plant. Biology, 5 (1). 812.
Dipak, K and H. Soma (2010). Plant breeding Biometry Biotechnology . New Central Book Agency (P) Ltd . London ,Delhi Kolkata
Grabowska, B. and K. Mynett(1970). Induction of changes in garden gladiolus, (Gladiolus hybridus. Hort.) under the influence of gamma rays Co60 (Russian). Biul. Instytutu Hodowii i Aklimatyzacji Roslin(IHAR), 1-2 : 85-8.
Kiong, A., A. Ling Pick, S.H. Grace Lai and A.R. Harun (2008). Physiological responses of Orthosiphon stamineus plantlets to gamma irradiation. Am-Eurasian J. Sustain. Agric. 2(2):135-149.
Kyambadde, J., F. Kansiime, L. Gumaelius, and G. Dalhammar (2004). A comparative study of Cyperus papyrus and Miscanthidium violaceum based constructed wetlands for wastewater treatment in a tropical climate. Water Research, 38(2):475-485.
Mirzahoseini H, E. Omidinia, M.Shams-Ghahfarokhi , G. Sadeghi and M. Razzaghi-Abyaneh (2009). Application of PCR-RFLP to rapid identification of the main pathogenic dermatophytes from clinical specimens. Iranian J. Publ Health. , 38(1):18-24.
Mahmoud, A.E.K (2013). Vegetable plants physiology. El-Maaref Publishers .Alexandria
Moran, R. ( 1982). Formula determination of chlorophyll pigment extracted with N,N diethyl formamide . Plant Physiology, 69 : 1376-1381.
Preussa, S.B. and A.B. Britta (2003). A DNA-damage-induced cell cycle checkpoint in Arabidopsis.Genetics, 164: 323-334.
Sax, K. (1955). The effect of ionizing radiation on plant growth. Amer. J. Bot., 42 : 360-364.
Sedel’nikova, L. (1988). Mutations in flower colour of gladioli from gamma irradiated corms. Byulleten' Glavnogo Botanicheskogo Sada, 148: 55-60.
Shaw J., Lickey E. B., Schilling E. E. and R.L. Small. ( 2007). Comparison of whole chloroplast genome sequences to choose noncoding regions for phylogenetic studies in angiosperms: The tortoise and the hare III. American Journal of Botany 94: 275–288.
Snedecor , G. W. and W. Cochran (1989). Statistical Methods, 8th ed. Iowa State University Press.
Van Harten , A.M. (1998). Mutation Breeding: Theory and Practical Applications. Cambridge University Press, Cambridge, UK.
Wi, S.G., B.Y. Chung, J.S. Kim , J.H. Kim , M.H. Baek , J.W. Lee and Y.S. Kim (2007). Effects of gamma irradiation on morphological changes and biological responses in plants. Micron, 38: 553-564.
Yadav V. (2016 ). Effect of gamma radiation on various growth parameters and biomass of Canscora decurrens Dalz . Inter.J. of Herbal Medicine; 4(5): 109-115 | ||||
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