Molecular Detection of Biogenic Bacteria During Biogas Production Using Domestic Feed Stock | ||||
| Egyptian Academic Journal of Biological Sciences, G. Microbiology | ||||
| Article 3, Volume 10, Issue 1, June 2018, Page 37-45 PDF (237.16 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/eajbsg.2018.16312 | ||||
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| Authors | ||||
| Anyakorah C. I.; Amoo I.; Obi C. C. | ||||
| Department of Biological Sciences, Bell's University of Technology Ota, Ogun State, Nigeria | ||||
| Abstract | ||||
| The search for a sustainable and environmental friendly energy has resulted to the development of an alternative renewable energy such as biogas. The objective of this work was to identify the presence of methanogenic bacteria in a short-term operated biogas reactor fed with kitchen waste by PCR-amplification of the methyl coenzyme M reductase (mcrA). Household organic waste was obtained from Bells University of Technology canteen while pig manure that served as the inoculum was obtained from a pig farm. Three replicate samples were taken every week and analysed for changes in the physico-chemical parameters to determine their effects on the rate of biogas production. Results obtained showed that the % moisture and carbon contents, before and after fermentation, were reduced from 88% to 80% and 16.7% to 12.5%, while % nitrogen content increased from 1.20% to 1.70%. The mean weekly biogas production ranged from 27.4 ml to 147 ml at 4 weeks and 6 weeks, respectively. Methyl coenzyme M reductase (mcrA) gene, which codes for the enzyme that catalyse the terminal step in methane production during the anaerobic fermentation of biomass, was amplified both in the methanogenic bacteria and transformed E. coli with the fragment size of 500 bp. | ||||
| Keywords | ||||
| Biogas. Methanogenic bacteria. Methyl coenzyme M reductase. PCR; amplification. Anaerobic digestion | ||||
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