Identification of Endoglucanase Gene Responsible for Cellulose Degradation Using Aspergillus flavus
El-Bakary, M., ElBaghdady, K., Ageez, A. (2018). Identification of Endoglucanase Gene Responsible for Cellulose Degradation Using Aspergillus flavus. EKB Journal Management System, 10(1), 47-57. doi: 10.21608/eajbsg.2018.16313
Mustafa El-Bakary; Khaled ElBaghdady; Amr Ageez. "Identification of Endoglucanase Gene Responsible for Cellulose Degradation Using Aspergillus flavus". EKB Journal Management System, 10, 1, 2018, 47-57. doi: 10.21608/eajbsg.2018.16313
El-Bakary, M., ElBaghdady, K., Ageez, A. (2018). 'Identification of Endoglucanase Gene Responsible for Cellulose Degradation Using Aspergillus flavus', EKB Journal Management System, 10(1), pp. 47-57. doi: 10.21608/eajbsg.2018.16313
El-Bakary, M., ElBaghdady, K., Ageez, A. Identification of Endoglucanase Gene Responsible for Cellulose Degradation Using Aspergillus flavus. EKB Journal Management System, 2018; 10(1): 47-57. doi: 10.21608/eajbsg.2018.16313

Identification of Endoglucanase Gene Responsible for Cellulose Degradation Using Aspergillus flavus

Egyptian Academic Journal of Biological Sciences, G. Microbiology
Article 4, Volume 10, Issue 1, June 2018, Page 47-57  XML PDF (607.39 K)
Document Type: Original Article
DOI: 10.21608/eajbsg.2018.16313
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Authors
Mustafa El-Bakary1; Khaled ElBaghdady2; Amr Ageez3
1Agricultural Genetic Engineering Research Institute, Agricultural Research Center, Giza, Egypt
2Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt
3Faculty of Biotechnology, October University for Modern Sciences and Arts (MSA), Egypt
Abstract
Twenty
two of cellulose-degrading fungal isolates were isolated from five different
samples; rotted sugar cane bagasse, rotted plant, termites, soil and animal
manure. Out of 22 isolates, 21 strains showed hydrolyzing zone on agar plates
containing carboxy methyl cellulose after iodine staining. The fungal isolate
No. S4, exhibited the highest endoglucanase (CMCase) activity with (0.165
IU/ml) in cellulase production culture. The best
exoglucanase (FPase) and endoglucanase (CMCase) of fungal isolate S4 was
obtained after incubation at 30°C for 7 days. Sugar cane bagasse (SCB) induced
the production of FPase and CMCase with maximum activity of 3.6 fold and 2.2
fold, respectively more than that of the maximum yield in case of
carboxy methyl cellulose (CMC). The fungal isolate S4 was identified as Aspergillus
flavus on the basis of 18S rRNA and ITS region sequence analysis.
Keywords
Aspergillus flavus; cellulose degradation
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