Purification and Characterization of Fibrinolytic Enzyme Produced by Bacillus subtilis Egy.

Elgamal, Nora N.; El- Beih, Fawkia; Hassnin, Saadia M.; Moharam, Maysa E.; El-Bendary, Magda A.; Abo Elsoud, Mostafa M.

doi:10.21608/eajbsg.2018.16314

	Purification and Characterization of Fibrinolytic Enzyme Produced by Bacillus subtilis Egy.
Egyptian Academic Journal of Biological Sciences, G. Microbiology
Article 5, Volume 10, Issue 1, June 2018, Page 59-68 PDF (514.42 K)
Document Type: Original Article
DOI: 10.21608/eajbsg.2018.16314
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Authors
Nora N. Elgamal¹; Fawkia El- Beih²; Saadia M. Hassnin²; Maysa E. Moharam¹; Magda A. El-Bendary¹; Mostafa M. Abo Elsoud³
¹Microbial Chemistry Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, Egypt
²Faculty of Science, Ain Shams University, Cairo, Egypt
³Microbial Biotechnology Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, Egypt
Abstract
A novel extracellular enzyme with strong fibrinolytic activity, produced by Bacillus subtilis Egy, which was isolated from the Egyptian soil was purified and characterized. The enzyme was secreted by cultured B. subtilis Egy under solid state fermentation and purified 21.5 purification fold using precipitation by ammonium sulphate followed by ion exchange chromatography, and gel filtration on Sephadex G- 100 chromatography. Purified enzyme showed optimum activity at 50^oCreaction temperature and pH 8. Enzyme stability studies revealed that the enzymeis stable up to 30 °C and it retained 80% of its activity at 40^oC.In addition, the purified enzyme is stable at pH 8-9. The fibrinolytic activity was enhanced by Mn^2+,Cu²⁺, Ca²⁺, Na⁺, and Ba²⁺ and inhibited by, Zn²⁺, and Hg²⁺. Moreover, the activity was decreased by EDTA and PMSF at 2 mM final concentration.
Keywords
Bacillus subtilis Egy; Fibrinolytic enzyme


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