Isolation and Characterization of Bacillus subtilis MF375213.1 and Bacillus axarquiensis MF375110.1 From Used Petroleum Oils at Assiut City, Egypt | ||||
| Egyptian Academic Journal of Biological Sciences, G. Microbiology | ||||
| Article 3, Volume 9, Issue 1, June 2017, Page 31-37 PDF (258.48 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/eajbsg.2017.16341 | ||||
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| Author | ||||
| Elsayed K. Bakhiet | ||||
| Botany and Microbiology Department, Faculty of Science, Al-Azhar University, 71524 Assiut, Egypt | ||||
| Abstract | ||||
| Petroleum oil is the main pollutant specially in petroleum countries producers. In This research study looked for bacterial strains have ability to degrade petroleum oily pollutants. Two bacterial isolates from 23 isolates have high ability to degrade engine oil were characterized and identified. The two bacterial isolates have been identified phenotypically and genotypically methods, as Bacillus subtilis strain BS1 and Bacillus axarquiensis strain BA1, these deposited in national center for biotechnology information (NCBI Gen Bank) under the Accession numbers MF375213.1 and MF375110.1 respectively. Two isolated strains have ability to grow in minimal medium containing petroleum oil with maximum bacterial growth was determined by spectrophotometry. Bacillus subtilis growth exhibited 1.7 OD at 600nm after 2 days of incubation time whereas Bacillus axarquiensis exhibited 1.14 OD at 600 nm after 3 days of incubation time with minimal medium containing 2% petroleum oil. | ||||
| Keywords | ||||
| Petroleum oils; Bacillus subtilis; Bacillus axarquiensis | ||||
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