Protein patterns of Admiral- treated and untreated house dust mites: Dermatophagoides pteronyssinus and Dermatophagoides farinae (Acarina: Pyroglyphidae)
Helmy, N., Abdel-Hamid, Y., Awad, A., Kenawy, M., El-Shafei, A., El-Zohery, Y. (2010). Protein patterns of Admiral- treated and untreated house dust mites: Dermatophagoides pteronyssinus and Dermatophagoides farinae (Acarina: Pyroglyphidae). EKB Journal Management System, 2(1), 33-39. doi: 10.21608/eajbse.2010.16457
Nadia Helmy; Yousrya M. Abdel-Hamid; Ashraf A. Awad; Mohamed A. Kenawy; Akila M. El-Shafei; Yousry Z. A. El-Zohery. "Protein patterns of Admiral- treated and untreated house dust mites: Dermatophagoides pteronyssinus and Dermatophagoides farinae (Acarina: Pyroglyphidae)". EKB Journal Management System, 2, 1, 2010, 33-39. doi: 10.21608/eajbse.2010.16457
Helmy, N., Abdel-Hamid, Y., Awad, A., Kenawy, M., El-Shafei, A., El-Zohery, Y. (2010). 'Protein patterns of Admiral- treated and untreated house dust mites: Dermatophagoides pteronyssinus and Dermatophagoides farinae (Acarina: Pyroglyphidae)', EKB Journal Management System, 2(1), pp. 33-39. doi: 10.21608/eajbse.2010.16457
Helmy, N., Abdel-Hamid, Y., Awad, A., Kenawy, M., El-Shafei, A., El-Zohery, Y. Protein patterns of Admiral- treated and untreated house dust mites: Dermatophagoides pteronyssinus and Dermatophagoides farinae (Acarina: Pyroglyphidae). EKB Journal Management System, 2010; 2(1): 33-39. doi: 10.21608/eajbse.2010.16457

Protein patterns of Admiral- treated and untreated house dust mites: Dermatophagoides pteronyssinus and Dermatophagoides farinae (Acarina: Pyroglyphidae)

Egyptian Academic Journal of Biological Sciences, E. Medical Entomology & Parasitology
Article 3, Volume 2, Issue 1, December 2010, Page 33-39  XML PDF (219.13 K)
Document Type: Original Article
DOI: 10.21608/eajbse.2010.16457
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Authors
Nadia Helmy1; Yousrya M. Abdel-Hamid2; Ashraf A. Awad3; Mohamed A. Kenawy3; Akila M. El-Shafei3; Yousry Z. A. El-Zohery4
1Department of Entomology, Faculty of Science, Ain Shams University.
2Research Institute of Medical Entomology, Ministry of Health
3Department of Entomology, Faculty of Science, Ain Shams University
4Department of Haematology and Immunology, Faculty of Medicine, Al-Azhar University, Cairo, A.R. Egypt
Abstract
The present work deals with the study of the effect of Admiral "pyriproxyfen” as a juvenile hormone analogue (JHA) on the protein patterns of the two common house dust mites (HDM): Dermatophagoides pteronyssinus and D. farinae.  Analysis revealed lower protein content in either JHA-treated or untreated tritonymphs than in the respective adults. JHA treatment affected protein concentration of both tritonymphs (significantly increased) and adults (significantly decreased). Significantly lower protein contents were detected for D. farinae than for D.pteronyssinus either in normal or JHA-treated individuals. A total of 49 bands with molecular weights of 10-108 Kilo Daltons (KD) and relative mobilities of 0.99-0.16 were electrophoretically separated from the whole body tissue extracts of normal and treated tritonymphs and adults of the two species. Four common bands (12-60 KD) were suggested as genus-specific. Three D. pteronyssinus proteins (21-33 KD) and one D. farinae protein (69 KD) were suggested as species- specific. The HDM allergen (14-15 KD) was detected in the two mite species. Several proteins that detected in normal individuals of the two species disappeared in the respective treated individuals that may be due to the effect of treatment with such JHA.
Keywords
house dust mites; Dermatophagoides pteronyssinus; D. farina; Protein%; Juvenile hormone analogues; Pyriproxyfen; Admiral
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