Incidence of Human Cytomegalovirus Viremia among Egyptian Hepatitis C - Patients with Hepatocellular Carcinoma | ||||
| Egyptian Academic Journal of Biological Sciences, G. Microbiology | ||||
| Article 2, Volume 8, Issue 2, December 2016, Page 11-21 PDF (659.18 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/eajbsg.2016.16471 | ||||
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| Authors | ||||
| Ahmed Khedr1; Marwa K. Ibrahim1; Ahmed B. Barakat2; Mohamed S. Salama3; Kouka S. Abdel-wahab4; Mostafa K. El-Awady5 | ||||
| 1Department of Microbial biotechnology, Genetic Engineering Division, National Research Centre, Cairo Egypt | ||||
| 2Department of Microbiology, Faculty of Science, Ain Shams University, Cairo, Egypt | ||||
| 3Molecular biology Laboratory, Faculty of Science, Ain Shams University. | ||||
| 4Department of Microbiology, Faculty of Medicine, Al-Azhar University,Cairo, Egypt | ||||
| 5Department of Microbial biotechnology, Genetic Engineering Division, National Research Centre, Cairo Egypt. | ||||
| Abstract | ||||
| Background: Hepatocellular carcinoma (HCC) is the major hepatic complication that may arise after many years of hepatitis C virus (HCV) infection. In Egypt, HCV represents the major health problem, also human cytomegalovirus (HCMV) is known as one of the highest un-resolved latent infections among general population. HCMV viremia in the co-infection with HCV may cause life threatening in HCC patients. Our study aimed to detect HCMV viremia in HCV–patients experienced HCC, and to investigate its role in disease worsening. Methods: In HCC patients, HCV-RNA viral load was determined by real- time polymerase chain reaction. In HCV-HCC patients, HCMV-DNA was detected by amplification of gB gene region using nested- polymerase chain reaction. Results: HCMV-DNA was detected in 4/73 in control subjects with prevalence rate of 5.4%, whereas HCMV–DNA was recorded in 24/75 HCV-HCC patients with prevalence rate of 32 %. Data on the level of alpha feto protein (AFP) was available for 59 out of 75 HCV-HCC patients, this enabled us to differentiate between low risk HCC group of 40/59 patients with AFP < 500 ng/ml, from them HCMV- DNA was detected in 14/40 patients with prevalence rate 35%, and high risk HCC group of 19/59 patients with AFP > 500 ng /ml, from them HCMV-DNA was reported in 9/19 patients with prevalence rate 47.37%. High significant prevalence rate of HCMV-DNA (P < 0.001) among control and HCC subjects was reported. Significant change in HCMV – DNA prevalence between low and high risk HCC groups could not be achieved but tendency of higher prevalence rate in HCMV- DNA was observed towards HCC high risk group of patients. Conclusion: we illustrated information about HCMV/ HCV co-infection in HCC patients, which referred to the association of HCMV viremia with HCV-HCC patients, as well as the tendency of elevation in HCMV viremia from low to high risk HCC patients depending on AFP threshold of 500 ng/ml. | ||||
| Keywords | ||||
| human cytomegalovirus; Hepatitis C virus; Hepatocellular carcinoma | ||||
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