Biochemical studies of the effect of arsenic trioxide on Ehrlich ascites tumor: Thesis Abstract
Shalaan, E., Salama, A., Ibrahim, W., Mansour, M. (2021). Biochemical studies of the effect of arsenic trioxide on Ehrlich ascites tumor: Thesis Abstract. EKB Journal Management System, 5(0), 26-26. doi: 10.21608/jcbr.2021.60584.1152
Eman Salah Shalaan; Afrah Fatthi Salama; Wafaa Mohammed Ibrahim; Mohammed Ahmed Mansour. "Biochemical studies of the effect of arsenic trioxide on Ehrlich ascites tumor: Thesis Abstract". EKB Journal Management System, 5, 0, 2021, 26-26. doi: 10.21608/jcbr.2021.60584.1152
Shalaan, E., Salama, A., Ibrahim, W., Mansour, M. (2021). 'Biochemical studies of the effect of arsenic trioxide on Ehrlich ascites tumor: Thesis Abstract', EKB Journal Management System, 5(0), pp. 26-26. doi: 10.21608/jcbr.2021.60584.1152
Shalaan, E., Salama, A., Ibrahim, W., Mansour, M. Biochemical studies of the effect of arsenic trioxide on Ehrlich ascites tumor: Thesis Abstract. EKB Journal Management System, 2021; 5(0): 26-26. doi: 10.21608/jcbr.2021.60584.1152

Biochemical studies of the effect of arsenic trioxide on Ehrlich ascites tumor: Thesis Abstract

Egyptian Journal of Cancer and Biomedical Research
Article 28, Volume 5, Issue 0, May 2021, Page 26-26  XML PDF (189.62 K)
Document Type: Essay Abstract
DOI: 10.21608/jcbr.2021.60584.1152
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Authors
Eman Salah Shalaan email 1; Afrah Fatthi Salama2; Wafaa Mohammed Ibrahim3; Mohammed Ahmed Mansour4
1Biochemistry division, chemistry department, faculty of science, Tanta university , Egypt
2Professor of Biochemistry Biochemistry Section, Chemistry Department, Faculty of Science, Tanta University
3Professor of Medical Biochemistry, Faculty of Medicine Tanta University
4Lecturer of Biochemistry Biochemistry Section, Chemistry Department, Faculty of Science Tanta University
Abstract
Thesis Abstract
Hexokinase 2 enzyme catalyzes the rate-limiting step in glycolysis. It is overexpressed in several carcinomas including breast cancer to sustain energy for rapidly dividing cells and associates with chemoresistance. However, impact of chemo drugs alone or in combination on hexokinase activity and autophagic cell death is unclear. Arsenic trioxide (As2O3) (ATO) is a metalloid with potent antineoplastic effects in several types of cancer especially acute promyelocytic leukemia and breast cancer. Due to the cytotoxicity and low efficacy of ATO when it is used alone, a combination of ATO with other chemotherapeutic drugs may provide a rational basis for novel therapeutic combinations. Despite these efforts, the specific cellular events that account for differential effects of ATO on cancer cells’viability and the possible therapeutic combinations remain to be well‐defined. In this report, we used an in vivo murine adenocarcinoma model to validate the effects of As2O3 and cisplatin on hexokinase activity and autophagic cancer cell death. We found that the two drugs inhibit hexokinase activity and induceautophagic marker, beclin 1 expression. Interestingly, combining As2O3 with cisplatin synergistically enhanced these effects and alleviated oxidative stress often encountered in As2O3 treatment. Altogether, our data provide direct evidence that inhibition of hexokinase activity and induction of autophagic cell death are mediating the antineoplastic effects of As2O3 and cisplatin. Our findings raise the potential of combiningAs2O3 with cisplatin as an approach to augment cisplatin-induced cell death and combat cisplatin chemo-resistance in cancer.
Keywords
Arsenic Trioxide; Autophagy; Beclin 1; Cisplatin; Hexokinase
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