Preparation and evaluation of graphene oxide based–materials for anticancer drug delivery in an experimental mice tumor: Thesis Abstract
Assy, L., Gemeay, A., Okba, S., Salem, M. (2021). Preparation and evaluation of graphene oxide based–materials for anticancer drug delivery in an experimental mice tumor: Thesis Abstract. EKB Journal Management System, 5(1.1), 9-9. doi: 10.21608/jcbr.2021.61754.1167
Lobna Assy; Ali Gemeay; Soha Okba; Mohamed Labib Salem. "Preparation and evaluation of graphene oxide based–materials for anticancer drug delivery in an experimental mice tumor: Thesis Abstract". EKB Journal Management System, 5, 1.1, 2021, 9-9. doi: 10.21608/jcbr.2021.61754.1167
Assy, L., Gemeay, A., Okba, S., Salem, M. (2021). 'Preparation and evaluation of graphene oxide based–materials for anticancer drug delivery in an experimental mice tumor: Thesis Abstract', EKB Journal Management System, 5(1.1), pp. 9-9. doi: 10.21608/jcbr.2021.61754.1167
Assy, L., Gemeay, A., Okba, S., Salem, M. Preparation and evaluation of graphene oxide based–materials for anticancer drug delivery in an experimental mice tumor: Thesis Abstract. EKB Journal Management System, 2021; 5(1.1): 9-9. doi: 10.21608/jcbr.2021.61754.1167

Preparation and evaluation of graphene oxide based–materials for anticancer drug delivery in an experimental mice tumor: Thesis Abstract

Egyptian Journal of Cancer and Biomedical Research
Article 11, Volume 5, Issue 1.1, May 2021, Page 9-9  XML PDF (181.06 K)
Document Type: Essay Abstract
DOI: 10.21608/jcbr.2021.61754.1167
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Authors
Lobna Assy email 1; Ali Gemeay2; Soha Okba3; Mohamed Labib Salemorcid 3
1Department of Zoology, Faculty of Science, Tanta University, Egypt
2Chemistry Department, Faculty of Science, Tanta University, Tanta 31527, Egypt.
3Immunology and Biotechnology Division, Department of Zoology, Faculty of Science, Tanta University, Egypt
Abstract
Thesis Abstract
Background: Graphene oxide (GO) is a multifunctional carbon nanomaterial with tremendous potential in medical science including cancer therapy. It has unique physical, and chemical properties to be used as a drug carrier such as Doxorubicin (DOX). Aim: This study aimed to load DOX on (GO) and supramagnetic iron oxide GO (GO/Fe3O4) as a passive and active forms with or without folic acid (FA) and to compare the anti-tumor effects of these conjugates to free DOX.
Materials and Methods: GO was synthesized by Hummers method, then loaded with DOX, FA or Fe3O4. All conjugates were characterized by FT-IR, TEM and TGA techniques, then their anticancer properties were investigated in vitro using EAC cell lines. In vivo study was performed using EAC-bearing mice which were divided and treated with DOX, GO/DOX, rGO/DOX/FA, GO/Fe3O4/DOX, rGO/Fe3O4/FA/DOX, GO/Fe3O4/DOX+IR and rGO/Fe3O4/FA/DOX+IR. After 10 days, number of tumor cells, splenocytes and white blood cells (WBC), apoptosis, and cell cycle of tumor cells were analyzed. Results: In vivo results showed that GO conjugates induced significant decrease of the total numbers of EAC cells. Interestingly rGO/Fe3O4/FA/DOX+ IR treatment showed increases in late apoptosis whereas GO/DOX and rGO/Fe3O4/FA/DOX induced necrotic cells as compared to free DOX. Free DOX induced leukopenia in spleen, however treatment with GO/DOX or GO/FA/DOX induced lesser effects. Treatment with GO/DOX conjugates induced significant increases in the blood leukocytes as compared to treatment with DOX and GO/DOX/FA which induced leukopenia. Conclusion: These results demonstrate that GO composites may be a highly biocompatible nanomaterial with practical applications in cancer therapy.
Keywords
Cancer; Doxorubicin; Ehrlich ascites; Folic acid, Graphene oxide
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