Enhancing the therapeutic chemosensitivity of hepatocellular carcinoma cells using alpha-solanine: Thesis Abstract
Gouhar, S., Abd Elhameed, F., Ashour, M., Hassan, N., El-Daly, S. (2021). Enhancing the therapeutic chemosensitivity of hepatocellular carcinoma cells using alpha-solanine: Thesis Abstract. EKB Journal Management System, 5(0), 24-24. doi: 10.21608/jcbr.2021.63663.1179
Shaimaa Abdelsadek Gouhar; Fatma Farag Abd Elhameed; Magdi Nabih Ashour; Nahla Samir Hassan; Sherien Mohamed El-Daly. "Enhancing the therapeutic chemosensitivity of hepatocellular carcinoma cells using alpha-solanine: Thesis Abstract". EKB Journal Management System, 5, 0, 2021, 24-24. doi: 10.21608/jcbr.2021.63663.1179
Gouhar, S., Abd Elhameed, F., Ashour, M., Hassan, N., El-Daly, S. (2021). 'Enhancing the therapeutic chemosensitivity of hepatocellular carcinoma cells using alpha-solanine: Thesis Abstract', EKB Journal Management System, 5(0), pp. 24-24. doi: 10.21608/jcbr.2021.63663.1179
Gouhar, S., Abd Elhameed, F., Ashour, M., Hassan, N., El-Daly, S. Enhancing the therapeutic chemosensitivity of hepatocellular carcinoma cells using alpha-solanine: Thesis Abstract. EKB Journal Management System, 2021; 5(0): 24-24. doi: 10.21608/jcbr.2021.63663.1179

Enhancing the therapeutic chemosensitivity of hepatocellular carcinoma cells using alpha-solanine: Thesis Abstract

Egyptian Journal of Cancer and Biomedical Research
Article 26, Volume 5, Issue 0, May 2021, Page 24-24  XML PDF (191.96 K)
Document Type: Essay Abstract
DOI: 10.21608/jcbr.2021.63663.1179
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Authors
Shaimaa Abdelsadek Gouhar email orcid 1; Fatma Farag Abd Elhameed2; Magdi Nabih Ashour1; Nahla Samir Hassan3; Sherien Mohamed El-Daly4
1Medical Biochemistry Department, Medical Research Division, National Research Centre, Dokki, 12622, Cairo, Egypt
2Department, Faculty of Science, Ain Shams University, Cairo, Egypt
3Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt
41-Medical Biochemistry Department, Medical Research Division, National Research Centre, Dokki, 12622, Cairo, Egypt 2-Cancer Biology and Genetics Laboratory, Centre of Excellence for Advanced Sciences, National Research Centre, Dokki 12622, Cairo, Egypt;
Abstract
Thesis Abstract
Background: Anti-cancer chemotherapy although effective it inducesserious  adverse effects. In addition, cancer cells can develope resistance to chemotherapy. Aim: The clinical application of cisplatin is limited by severe side effects associated with high applied doses. The synergistic effect of a combination treatment of a low dose of cisplatin with the natural alkaloid α-solanine on human hepatocellular carcinoma cells was evaluated. Materials and Methods: HepG2 cells were exposed to low doses of α-solanine and cisplatin, either independently or in combination. The efficiency of this treatment modality was evaluated by investigating cell growth inhibition, cell cycle arrest, and apoptosis enhancement. Results: α-solanine synergistically potentiated the effect of cisplatin on cell growth inhibition and significantly induced apoptosis. This synergistic effect was mediated by inducing cell cycle arrest at the G2/M phase, enhancing DNA fragmentation and increasing apoptosis through the activation of caspase 3/7 and/or elevating the expression of the death receptors DR4 and DR5. The induced apoptosis from this combination treatment was also mediated by reducing the expression of the anti-apoptotic mediators Bcl-2 and survivin, as well as by modulating the miR-21 expression. Conclusion: Our study provides strong evidence that a combination treatment of low doses of α-solanine and cisplatin exerts a synergistic anticancer effect and provides an effective treatment strategy against hepatocellular arcinoma.
Keywords
Cisplatin; Bcl-2; HepG2 cells; miR-21; α-solanine
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