Histopathological Impact of Ginger Loaded Nanoparticle Versus Ginger Extract as A Novel Therapy of Experimentally Induced Acute Ulcerative Colitis
AL Badawi, M., Waly, N., Eid, M., Soliman, N. (2022). Histopathological Impact of Ginger Loaded Nanoparticle Versus Ginger Extract as A Novel Therapy of Experimentally Induced Acute Ulcerative Colitis. EKB Journal Management System, 45(2), 442-456. doi: 10.21608/ejh.2021.68124.1448
Manal H. AL Badawi; Nermien E. Waly; May M. Eid; Nahed A. Soliman. "Histopathological Impact of Ginger Loaded Nanoparticle Versus Ginger Extract as A Novel Therapy of Experimentally Induced Acute Ulcerative Colitis". EKB Journal Management System, 45, 2, 2022, 442-456. doi: 10.21608/ejh.2021.68124.1448
AL Badawi, M., Waly, N., Eid, M., Soliman, N. (2022). 'Histopathological Impact of Ginger Loaded Nanoparticle Versus Ginger Extract as A Novel Therapy of Experimentally Induced Acute Ulcerative Colitis', EKB Journal Management System, 45(2), pp. 442-456. doi: 10.21608/ejh.2021.68124.1448
AL Badawi, M., Waly, N., Eid, M., Soliman, N. Histopathological Impact of Ginger Loaded Nanoparticle Versus Ginger Extract as A Novel Therapy of Experimentally Induced Acute Ulcerative Colitis. EKB Journal Management System, 2022; 45(2): 442-456. doi: 10.21608/ejh.2021.68124.1448

Histopathological Impact of Ginger Loaded Nanoparticle Versus Ginger Extract as A Novel Therapy of Experimentally Induced Acute Ulcerative Colitis

Egyptian Journal of Histology
Article 9, Volume 45, Issue 2, June 2022, Page 442-456  XML PDF (1.29 MB)
Document Type: Original Article
DOI: 10.21608/ejh.2021.68124.1448
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Authors
Manal H. AL Badawi email orcid 1; Nermien E. Waly2; May M. Eid3; Nahed A. Soliman4
1Anatomy & Embryology Department, faculty of medicine Helwan University, Egypt.
2Physiology Department, faculty of medicine ,Helwan university
3Spectroscopy Department (Physics Division), National Research Centre, Dokki, Giza,12311, Egypt.
4Pathology Department. faculty of medicine Helwan University, Egypt.
Abstract
Background: Ulcerative colitis (UC) is a debilitating chronic inflammatory bowel disease that is prevalent in Egypt. Current treatment of UC is expensive and has serious side effects.
Aim of the Work: To evaluate the effect of ginger loaded nanoparticles (GDNP) compared to ginger extract (GE) as an alternative treatment of acute UC (AUC) in DSS/ ethanol rat model.
Material and Methods: Twenty rats were used. AUC was induced by administration of 2% synthetic dextran sulfate sodium (DSS). Rats were divided into 4 groups: control (GP I), AUC (GP II), AUC received 2.5 gm GE (GP III) and AUC received 2.5 gm superparamagnetic@ silver nanoparticles GDNP (GP VI). Colonic and rectal tissue were assessed histologically using Hematoxylin and eosin (H&E), Periodic acid- Schiff (PAS), Toluidine blue, and electron microscopy (EM). Epithelial proliferation and apoptosis were assessed by anti-KI-67 antibody anti BCL2 antibody.
Results: GP III and GP IV showed improvement with GDNP and GE compared to control. Gross damage scores were as follows: GPI 0±0, GPII 2.6±1.1, GPIII 1.4±1.1, and GPIV 0±0 with significant variation (P = 0.000). Cumulative histopathological score of GPI, II, III, and IV were 0±0, 9.2±3.3, 3±1.6, and 1±1 respectively with significant correlation (P= 0.000). GPIII and GPIV displayed strong positive nuclear and cytoplasmic staining for KI-67 (GPIII Mean=50±15/ HPF). (GPIV Mean=66±8/ HPF) and BCL2 (Mean=60±16 and 76±9/ HPF) with significant variation (P= 0.000).
Conclusion: GDNP has more potential in treatment of AUC compared to GE. Treatment with GDNPs improved signs, reduced apoptosis and enhanced repair of AUC more significantly
Keywords
BCL2; ginger; ginger loaded nanoparticles; KI-67; ulcerative colitis
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