Laccase Production From Trametes hirsuta and Decolourisation of Phenolic Textile Dye In a Laccase Mediator System (LMS)
Taha, A., Al-Mudallal, N., Sadiq, G., Abdullatif, M., Glaiym, H., Stamatis, H., Dheeb, B. (2015). Laccase Production From Trametes hirsuta and Decolourisation of Phenolic Textile Dye In a Laccase Mediator System (LMS). EKB Journal Management System, 7(1), 19-27. doi: 10.21608/eajbsg.2015.16482
Ali A. Taha; Nada H. Al-Mudallal; Ghassaq Tariq Sadiq; Mohammed Omar Abdullatif; Haider Abdullzahra Glaiym; Haralambos Stamatis; Batol Imran Dheeb. "Laccase Production From Trametes hirsuta and Decolourisation of Phenolic Textile Dye In a Laccase Mediator System (LMS)". EKB Journal Management System, 7, 1, 2015, 19-27. doi: 10.21608/eajbsg.2015.16482
Taha, A., Al-Mudallal, N., Sadiq, G., Abdullatif, M., Glaiym, H., Stamatis, H., Dheeb, B. (2015). 'Laccase Production From Trametes hirsuta and Decolourisation of Phenolic Textile Dye In a Laccase Mediator System (LMS)', EKB Journal Management System, 7(1), pp. 19-27. doi: 10.21608/eajbsg.2015.16482
Taha, A., Al-Mudallal, N., Sadiq, G., Abdullatif, M., Glaiym, H., Stamatis, H., Dheeb, B. Laccase Production From Trametes hirsuta and Decolourisation of Phenolic Textile Dye In a Laccase Mediator System (LMS). EKB Journal Management System, 2015; 7(1): 19-27. doi: 10.21608/eajbsg.2015.16482

Laccase Production From Trametes hirsuta and Decolourisation of Phenolic Textile Dye In a Laccase Mediator System (LMS)

Egyptian Academic Journal of Biological Sciences, G. Microbiology
Article 3, Volume 7, Issue 1, December 2015, Page 19-27  XML PDF (267.61 K)
Document Type: Original Article
DOI: 10.21608/eajbsg.2015.16482
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Authors
Ali A. Taha1; Nada H. Al-Mudallal2; Ghassaq Tariq Sadiq3; Mohammed Omar Abdullatif3; Haider Abdullzahra Glaiym3; Haralambos Stamatis4; Batol Imran Dheeb5
1Applied Science Department, University of Technology, Baghdad, Iraq
2Department of Microbiology, College of Medicine, University of Al Iraqia, Baghdad, Iraq
3Biotechnology Research Center , University of Al Nahrain , Baghdad, Iraq
4Department of Biological Applications and Technologies, University of Ioannina, Ioannina, Greece
5Biology Department/ Iraqia University
Abstract
In
this study, fungal laccase was produced from Trametes
hirsuta DSMZ 5072 at 28 °C in a submerged
cultivation medium comprising: potato infusions from 200gm/L, glucose (1%) and malt
extract (1%). The maximum laccase activity
(129 U/L) was observed at day eight post-cultivation in the presence of 2mM of 2,5-xylidine, as an inducer. Fungal laccase from Tramates
versicolor as a commercial enzyme, and home prepared laccase from T. hirsuta, were used to decolourise phenol
red. The effects of initial medium pH, different
systems, type of mediator and incubation temperature were investigated. It was
observed that, for both commercial and homemade laccases, pH 4.5 allowed more
efficient decolourisation of the dye at 30 °C.
In an aqueous system, high decolourisation
percentages, of 42.8% and 36.4%, were revealed within 72 hours in the
presence of T. versicolor and T. hirsuta laccases respectively. The
degree of decolourisation increased in the aqueous system when a different
mediator was added. Results showed that the 1-hydroxybenzotriazole (HBT) has a much
better decolourisation ability than the other mediators tested, such that decolourisation
percentages as high as 73.6% and 79.4 were observed in the presence of 8mM HBT
and laccase from T. hirsuta (40 °C/120 minutes) or T. versicolor
(30 °C/120 minutes), respectively.
Keywords
Textile dye; Phenol red; Decolourisation; Laccase and LMS
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