MICROPROPAGATION OF EGYPTIAN DATE PALM C.V SELMY THROUGH FLORAL BUDS CULTURE. | ||||
| Journal of Plant Production | ||||
| Article 18, Volume 33, Issue 4, April 2008, Page 2803-2815 PDF (965.46 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2008.164923 | ||||
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| Author | ||||
| A. E. Hegazy* | ||||
| Genetic Engineering and Biotechnology Research Institute (GEBRI), Plant Biotech. Sec., Minufiya University, Sadat City, Egypt | ||||
| Abstract | ||||
| The totally closed date palm (Phoenix dactylifera L) female spathe was surface sterilized by sprayed with ethyl alcohol 70 % prior to aseptic conditions transfer, then flamed. To minimize fast oxidative browning, inflorescence stalks were shortened down to 3 cm then immersed in sterilized antioxidant solution containing citric and ascorbic acids 150 mg/L each for 2 hours, prior to culture. Modified MS medium supplemented with NOA (5 mg/L) + NAA (5 mg/L) + 2iP (1 mg/L) + BA (1 mg/L) was superior in explant survival %, callus formation %, creamy callus colour % and subsequently embryogenic callus formation % after 4 months of incubation. The modified medium (3/4 salts strength) supplemented with IBA (0.3 mg/L) and 2iP (0.5 mg/L) was recorded higher percentage values of embryos formation and the highest significant percentage values of number of embryos as well as embryos fresh weight (g) after 6 weeks of incubation. Embryos cultured on the previous medium in addition to putrescine (100 mg/L) obtained significant values of multiplication rate and growth value as well as total soluble protein and PAL activity after 3 weeks of incubation. Individual shootlets cultured on basal MS medium (3/4 salts strength) supplemented with putrescine (100 mg/L) and IBA (0.5 mg/L) achieved the highest significant values of root formation %, number of roots and root length (cm) as well as PAL activity after 2 months of incubation. On the other hand, using of soil culture containing compost and perlite (1:1, v/v) significantly recorded the highest survival percentage, number of leaves/plantlet and leaf length (cm) for plantlets after three months of acclimatization. | ||||
| Keywords | ||||
| Phoenix dactylifera L; embryogenesis; in vitro; tissue culture; inflorescence; PAL enzyme. TDZ (thidizuron); CPA (p-chlorophenoxyacetic acid); acclimatization | ||||
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