Evaluation of Saudi Fluorescent Pseudomonads Isolates as a Biocontrol Agent against Citrus Canker Disease Caused by Xanthomonas citri subsp citri A* | ||||
| Egyptian Academic Journal of Biological Sciences, G. Microbiology | ||||
| Article 1, Volume 6, Issue 2, December 2014, Page 1-7 PDF (146.35 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/eajbsg.2014.16493 | ||||
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| Author | ||||
| Mohammed A. Al-Saleh | ||||
| Plant Protection Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Kingdom of Saudi Arabia | ||||
| Abstract | ||||
| The goal of this study was to determine whether bacterial antagonists could be used to control Xanthomonas citri subsp citri (Xcc), the causal agent of bacterial citrus canker disease. A total of 22 potentially bacterial antagonists isolated as epiphytes from the phylloplane of healthy citrus trees were screened for their in vitro biological control capability against Xcc. These strains were identified as Pseudomonas fluorescens on the basis of biochemical and physiological tests and 16S rDNA. Out of these 22 potentially bacterial antagonists, five strains (KSA1, KSA9, KSA14, KSA17, and KSA20) showed high potential growth inhibition capabilities against Xcc. The KSA1 strain was selected for further studies to test its in vivo capability to control bacterial citrus canker pathogen. It was sprayed in a suspension of 107 CFU ml-1 on citrus leaves which were subsequently inoculated after 72 h with 108 CFU ml-1 suspension of Xcc strain JQ890095. According to the in vivo biocontrol tests, the putative antagonist KSA1 significantly reduced the symptoms on the leaves of Mexican lime seedlings compared with untreated inoculated plants. | ||||
| Keywords | ||||
| Citrus bacterial canker; biological control; Pseudomonas fluorescens | ||||
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