Mesoderm Signaling Pathways of Early Development in the Zebrafish Danio rerio | ||||
| Egyptian Journal of Aquatic Biology and Fisheries | ||||
| Article 33, Volume 25, Issue 2, March and April 2021, Page 601-614 PDF (707.75 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejabf.2021.165931 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Ilham J.J. Alshami; Hussein A. Saud | ||||
| Abstract | ||||
| The zebrafish (Danio rerio) is an ideal fish model for molecular and genetic studies. It is relatively easy to keep in the aquarium and to obtain embryos. The ability to follow embryonic development using microscopic and molecular techniques facilitates the study of mesoderm development. Hence, this study was conducted to investigate the role of four transcription factors that include: bone morphogenetic protein (BMP), nodal, fibroblast growth factor (FGF) and sonic hedgehog (SHH) in mesoderm induction, using specific inhibitors (DM, SB431542, SU5402) and Cyclopamine, respectively. Each inhibitor perturbed mesoderm development and in situ hybridisation, using myl10 specific marker for muscles. Results showed a noticeable reduction in gene expression with each treatment, indicating a crucial role for all four transcription factors in mesoderm development. | ||||
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