THERAPEUTIC AND IMMUNO-MODULATORY EFFECT OF SILVER NANOPARTICLES-LOADED WITH METRONIDAZOLE ON EXPERIMENTAL BLASTOCYSTOSIS | ||||
| Journal of the Egyptian Society of Parasitology | ||||
| Article 23, Volume 51, Issue 1, April 2021, Page 175-182 PDF (1.12 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jesp.2021.165964 | ||||
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| Authors | ||||
| DOAA A. AHMED; NAGWA M. KAMAL; AMAL A. MOHAMED; HALA E. ABDEL-HAMIED; KHADYGA H. ABDELFADIL | ||||
| Departments of Medical Parasitology, and Histopathology, Faculty of Medicine for Girls Al-Azhar University, Nasr City, Post Code: 11884, Egypt | ||||
| Abstract | ||||
| Blastocystis is one of the commonest enteric protozoa in many hosts. This study evaluated the effect of silver nanoparticles (AgNps) loaded with metronidazole (MTZ) on mice infected with Blastocystis. They were subjected to parasitological, histopathological, immunological, and immunohistochemical studies. The parasitological results revealed a powerful effect of Ag NPs loaded with MTZ over either MTZ or Ag Nps; where the reduction of cysts count in stool were (90. 7%, 87.33% & 59.33%) respectively as compared to infected non-treated group. Histopathological results showed that AgNPs loaded with MTZ were the best in reducing inflammatory changes followed by MTZ then AgNPs. Regarding IgA, IgM & IgG, Ag NPs loaded with MTZ treated mice showed the best result followed by MTZ treated mice then Ag NPs treated mice; where the reduction of mean concentration of IgA were (30.5%, 26.24% & 2.13%) respectively, while that of IgM were (15.2%, 12.8% & 2.8%) respectively and IgG were (23.1%, 12.3% & 5.2%) respectively as compared to infected non-treated group. The synergistic effect of both Ag NPS & MTZ provoked the best reduction of CD3 & CD20 expression and augmented counter modulation of the immune response to restore normal immune homeostasis. | ||||
| Keywords | ||||
| Blastocystis; Metronidazole; Silver nanoparticles; Experimental mice | ||||
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