ENZYMATIC ACTIVITIES OF FUNGI ISOLATED FROM LETTUCE LEAVES | ||||
| Journal of Plant Production | ||||
| Article 5, Volume 33, Issue 5, May 2008, Page 3383-3389 PDF (479.69 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2008.166396 | ||||
 View on SCiNiTO | ||||
| Author | ||||
| Hoda H. Al-Homeidan* | ||||
| Botany Department, College of Education-Riyadh University for Girls P.O. Box 27104, Riyadh 11417, Kingdom of Saudi Arabia | ||||
| Abstract | ||||
| Six fungal species, namely: Alternaria alternata, Botrytis cinerea, Fusarium rousm, F. tricnictum, Stemphylium botryosum and Thielaviopsis sp. were isolated from lettuce leaves collected from Riyadh markets during summer months in 2005. The fungal isolates were examined for their ability to produce five extracellular enzymes, namely: amylase, lipase, protease, cellulose and phenoloxidase, on solid media. They were also examined for production of one endoenzyme which is phosphatase. The results showed that all isolates were able to produce amylase though with variable magnitudes; the highest amylase activity was displayed by F.rousm followed by F. tricnictum. Concerning lipase activity Theilaviopsis sp. and F. rousm showed the highest potential; A. alternate and F. tricnictum came next, whereas B. cinerea and S. botryosum failed to produce lipase.Regarding protease enzyme, only two isolates (A. alternata and F. rousm) were able to produce the enzyme. The present study showed also that A. alternata followed by B. cineria, F. rousm and S. botryousm were able to produce cellulase, whereas the other two fugal species did not display any cellulase activity. On the other hand, all isolates, except B. cinerea do not produce phenoloxidase. Meanwhile, the largest colonial diameter on agar media was yielded by F .rousm followed by S. botryosum A. alternata, F. tricnictum, and B. cinerea, respectively. This investigation also showed that the cell-free extracts of all the fungal isolates tested appeared to catalyze the phosphate ( adenosine 5-monophosphate, AMP) hydrolysis; and the following sequence was displayed for acid phosphatase activity of the experimental fungal isolates: A. alterna, B. cinerea , S. botryousm, Theilaviopsis sp., F. tricnictum andF. rousm, in descending order, respectively. | ||||
| Keywords | ||||
| Alternaria alternata; Botrytis cinerea; Fusarium rousm; Fusarium tricnictum; Stemphylium botryosum; Thielaviopsis spp; amylase; lipase; protease; cellulase; phenoloxidase; phosphatase; lettuce | ||||
|
Statistics Article View: 79 PDF Download: 185 |
||||
