First Isolation, In Egypt, of a granulovirus (AgseGVEG) from Agrotis segetum Schiffermuller (Lepidoptera: Noctuidae) | ||||
| Egyptian Academic Journal of Biological Sciences, G. Microbiology | ||||
| Article 11, Volume 4, Issue 1, December 2012, Page 93-99 PDF (887.87 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/eajbsg.2012.16664 | ||||
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| Author | ||||
| Magda Khattab | ||||
| Plant Protection Research Institute, ARC, Dokki, Giza, Egypt Department of Cutworms and Mole Cricket | ||||
| Abstract | ||||
| A new Granulovirus was isolated from cutworm Agrotis segetum larvae collected from the field in Qalubaya governorate. Transverse section of larvae showed infected fat body, epidermis and mid-gut cells. Transmition electromicroscope evealed ovoid occlusion bodies (granules), each granule contains a single rod-shaped virion with one nucleocapsid, typical of granuolosis virus. Also, the scanning electromicroscopy of apurified viral suspension confirmed a baculovirus morphology. The size of virus particles in the present isolation ranged between 1.9 mm to 2.5mm. Preliminary bioassay indicated that A. ipsilon neonate larvae are susceptible to AgseGVEG and the calculated LC50 value was 3.79x106capsules/ml. | ||||
| Keywords | ||||
| Baculovirusisolation; cutworms; Agrotis segetum; granulovirus (AgseGVEG) | ||||
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