Validity of Antimycolic Acids Antibodies in the Diagnosis of Pulmonary Tuberculosis in TB/HIV Co-infected Patients in Khartoum State, Sudan
Elhassan, M., Elhassan, O., Dirar, A., Abbas, E., Elmekki, M. (2011). Validity of Antimycolic Acids Antibodies in the Diagnosis of Pulmonary Tuberculosis in TB/HIV Co-infected Patients in Khartoum State, Sudan. EKB Journal Management System, 3(1), 27-32. doi: 10.21608/eajbsg.2011.16692
Mogahid M Elhassan; Osama A. Elhassan; Ahmed E. Dirar; Elrayah E. Abbas; Miskelyemen A. Elmekki. "Validity of Antimycolic Acids Antibodies in the Diagnosis of Pulmonary Tuberculosis in TB/HIV Co-infected Patients in Khartoum State, Sudan". EKB Journal Management System, 3, 1, 2011, 27-32. doi: 10.21608/eajbsg.2011.16692
Elhassan, M., Elhassan, O., Dirar, A., Abbas, E., Elmekki, M. (2011). 'Validity of Antimycolic Acids Antibodies in the Diagnosis of Pulmonary Tuberculosis in TB/HIV Co-infected Patients in Khartoum State, Sudan', EKB Journal Management System, 3(1), pp. 27-32. doi: 10.21608/eajbsg.2011.16692
Elhassan, M., Elhassan, O., Dirar, A., Abbas, E., Elmekki, M. Validity of Antimycolic Acids Antibodies in the Diagnosis of Pulmonary Tuberculosis in TB/HIV Co-infected Patients in Khartoum State, Sudan. EKB Journal Management System, 2011; 3(1): 27-32. doi: 10.21608/eajbsg.2011.16692

Validity of Antimycolic Acids Antibodies in the Diagnosis of Pulmonary Tuberculosis in TB/HIV Co-infected Patients in Khartoum State, Sudan

Egyptian Academic Journal of Biological Sciences, G. Microbiology
Article 5, Volume 3, Issue 1, December 2011, Page 27-32  XML PDF (230.98 K)
Document Type: Original Article
DOI: 10.21608/eajbsg.2011.16692
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Authors
Mogahid M Elhassan1; Osama A. Elhassan2; Ahmed E. Dirar2; Elrayah E. Abbas3; Miskelyemen A. Elmekki1
1College of Medical Laboratory Science, Sudan University of Science and Technology
2Federal Ministry of Health
3Military Hospital, Omdurman, Sudan
Abstract
This study aimed to determine antimycolic acid antibodies [IgG and IgM] among TB/HIV Co-infected patients in Khartoum State. Sputum and blood specimens were collected from patients attended Alsha'ab Teaching Hospital, Tuberculosis Reference Laboratory, Ibrahim Malik Hospital and Abu Anga Hospital; patients were all informed and consented. Direct smears from sputum samples of 90 suspected patients showed that 17 (18.9%) were acid fast bacilli positive while 73 (81.1%) were negative. The ninety sputum specimens were subjected to PCR to amplify IS6110 region specific for Mycobacterium tuberculosis complex. The result showed that 79 (87.8%) were   positive for IS6110 while 11 (12.2%) were negative.  The 90 serum samples were investigated for HIV using dot plot technique, 9 samples (10%) were found HIV positive and they were all TB positive by PCR. 80 Serum samples were analyzed by ELISA, 16 (20%) gave positive result for antimycolic acid IgG while 64(80%) were negative and 55 (68.8%) were positive for antimycolic IgM, while 25 (31.3%) were negative. When HIV infection was correlated with specific antibodies for antimycolic acids, in 6 HIV positive samples, one (17 %) was positive and 5 (83 %) were negative for IgG, while 2 (33.3 %) were positive and 4 (67 %) were negative for IgM. This study concluded that patients with TB/HIV Co-infection have less capability of producing antimycolic acids antibodies, however, IgM antibodies could be of more serodiagnostic value.
Keywords
Mycolic acids; antibodies; HIV; tuberculosis; Sudan
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