Molecular study of Beta-Haemolytic Streptococci in Patients with Tonsillitis In Ramadi City
Al-Khalifawi, S., Al-Khafaji, A., Al-zobaei, M. (2011). Molecular study of Beta-Haemolytic Streptococci in Patients with Tonsillitis In Ramadi City. EKB Journal Management System, 3(1), 33-39. doi: 10.21608/eajbsg.2011.16693
Samira M. Al-Khalifawi; Alaa A. Al-Khafaji; Muthana A. Al-zobaei. "Molecular study of Beta-Haemolytic Streptococci in Patients with Tonsillitis In Ramadi City". EKB Journal Management System, 3, 1, 2011, 33-39. doi: 10.21608/eajbsg.2011.16693
Al-Khalifawi, S., Al-Khafaji, A., Al-zobaei, M. (2011). 'Molecular study of Beta-Haemolytic Streptococci in Patients with Tonsillitis In Ramadi City', EKB Journal Management System, 3(1), pp. 33-39. doi: 10.21608/eajbsg.2011.16693
Al-Khalifawi, S., Al-Khafaji, A., Al-zobaei, M. Molecular study of Beta-Haemolytic Streptococci in Patients with Tonsillitis In Ramadi City. EKB Journal Management System, 2011; 3(1): 33-39. doi: 10.21608/eajbsg.2011.16693

Molecular study of Beta-Haemolytic Streptococci in Patients with Tonsillitis In Ramadi City

Egyptian Academic Journal of Biological Sciences, G. Microbiology
Article 6, Volume 3, Issue 1, December 2011, Page 33-39  XML PDF (999.34 K)
Document Type: Original Article
DOI: 10.21608/eajbsg.2011.16693
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Authors
Samira M. Al-Khalifawi1; Alaa A. Al-Khafaji2; Muthana A. Al-zobaei2
1Medical Microbiology, Al-Ramadi Hospital
2Medical Microbiology, Microbiol. Dept., College of Medicine, University of Anbar
Abstract
Objectives: To evaluate the sensitivity and specificity of polymerase chain reaction PCR technique in early detection of infection with group A beta-haemplytic streptococci in comparing with conventional diagnostic methods among the patients with tonsillitis.
Methods: One hundred ninety one swabs and blood specimens were obtained from patients with tonsillitis in addition to samples of healthy control group during a period between 2008-2009 in general Ramadi hospital, Iraq and the tests (PCR) and other bacteriological and serological test at the same time on these samples.
Results: Our data show that in recurrent tonsillitis group out of 72 GBHS isolates 43 (59.7%) were detected by PCR, however 15(20.8%) were positive by serogrouping. Out of 75 bacterial isolates, 22 (29.4%) isolates were GABHS and 15 (83.5%) of these were GABHS detected by PCR and only 4 (5.3%) of GABHS detected by serogrouping methods in acute recurrent group, and our results revealed that the inoculation of penicillin and L-therionine to the brain heart infusion broth improved the DNA extraction from gram positive bacteria (GBHS).
Conclusion: Our study confirmed that the PCR technique more sensitive and highly specific in early detection of GBHS among the patients with tonsillitis in comparing with conventional; diagnostic methods.
Keywords
type A Streptococcus; PCR; Tonsillitis
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