Serological Detection of the Thermoactinomyces vulagris Antigen in Farmer’s lung disease Patients using ELISA method | ||||
| Egyptian Academic Journal of Biological Sciences, G. Microbiology | ||||
| Article 8, Volume 2, Issue 2, December 2010, Page 59-64 PDF (297.02 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/eajbsg.2010.16709 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Basima A. Abdulla; Essra G. Al-Sammak; Anmar A. Al-Taie | ||||
| Biology Dep., College of Science, Mousal University | ||||
| Abstract | ||||
| Serological detection of IgG antibodies to antigens one soil isolate 0f thermophilic actinomycetes belonged to the species Thermoactinomyces vulgaris in (70) sera of patients with farmer’s lung disease and hay fever patients compared with control normal peoples in the same environmental condition were carried out using ELISA method. The results indicated that (19) sera from symptomatic patients having IgG level between (1.007-1.626 ) I.U/ml, four of them having farmer’s lung disease and the remaining have hay fever as diagnosed by clinical . The IgG level between (0.293-0.944) I.U/ml indicated negative and which was found in(16) patients two of them having farmer’s lung disease and the remaning have hay fever. The IgG level of normal people were devided in two groups: first account positive control was between (1.089-2.147) I.U/ml and the second account negative was between (0.819-0.959) I.U/ml. The present study has demonstrated the usefulness of estimation of specific IgG antibodies activity to thermophilic actinomyces by ELISA for the diagnosis of farmer’s lung disease as a screening test of large number of samples with sufficient high sensitivity. | ||||
| Keywords | ||||
| Thermoactinomyces vulagris; antigen; lung disease; ELISA | ||||
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