Sensitivity of nested PCR toward human cytomegalovirus-DNA in native sera and in extracted DNA of serum samples
Shoman, S., Tabl, A., Ghanem, H., Nabil, M. (2010). Sensitivity of nested PCR toward human cytomegalovirus-DNA in native sera and in extracted DNA of serum samples. EKB Journal Management System, 2(1), 1-8. doi: 10.21608/eajbsg.2010.16711
Sahar Shoman; Ashraf Tabl; Hussam Ghanem; Mohamed Nabil. "Sensitivity of nested PCR toward human cytomegalovirus-DNA in native sera and in extracted DNA of serum samples". EKB Journal Management System, 2, 1, 2010, 1-8. doi: 10.21608/eajbsg.2010.16711
Shoman, S., Tabl, A., Ghanem, H., Nabil, M. (2010). 'Sensitivity of nested PCR toward human cytomegalovirus-DNA in native sera and in extracted DNA of serum samples', EKB Journal Management System, 2(1), pp. 1-8. doi: 10.21608/eajbsg.2010.16711
Shoman, S., Tabl, A., Ghanem, H., Nabil, M. Sensitivity of nested PCR toward human cytomegalovirus-DNA in native sera and in extracted DNA of serum samples. EKB Journal Management System, 2010; 2(1): 1-8. doi: 10.21608/eajbsg.2010.16711

Sensitivity of nested PCR toward human cytomegalovirus-DNA in native sera and in extracted DNA of serum samples

Egyptian Academic Journal of Biological Sciences, G. Microbiology
Article 1, Volume 2, Issue 1, June 2010, Page 1-8  XML PDF (454.41 K)
Document Type: Original Article
DOI: 10.21608/eajbsg.2010.16711
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Authors
Sahar Shoman1; Ashraf Tabl2; Hussam Ghanem1; Mohamed Nabil1
1Department of Microbiology, Faculty of Science, Ain Shams University, Cairo, Egypt
2Department of Biomedical Technology, National Research Center, Giza, Egypt
Abstract
            PCR has been commonly used for genomic viral diagnosis for its sensitivity and accuracy. It showed a higher sensitivity when compared to virus isolation in tissue culture and also in antigenemia detection. Definitely, DNA samples are critical factor in PCR validity. Out of 84 serum samples subjected to this study and extracted by Wizard® DNA purification mini kit, 27 samples (32.1%) were positive PCR. While, 15 samples (17.9%) only out of the same population study (84) were positive PCR for HCMV DNA in native serum samples (without DNA extraction). This result was confirmed the importance of DNA extraction from serum samples for detection of HCMV which, subsequently lead to more sensitive diagnostic tool of an ongoing HCMV infection.
Keywords
HCMV DNA; HCV RNA; nested PCR; DNA extraction; serum
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