Plasmid mediated virulence factors of some Proteus isolates | ||||
Egyptian Academic Journal of Biological Sciences, G. Microbiology | ||||
Article 2, Volume 1, Issue 1, December 2009, Page 7-22 PDF (276.37 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/eajbsg.2009.16713 | ||||
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Authors | ||||
Khaled Z. El-Baghdady1; Mohammad M. Abooulwafa2; Madeha O. Ghobashy3; Hassan M. G Gebreel3 | ||||
1Microbiology Department, Faculty of science, Ain Shams Univ., Cairo, Egypt | ||||
2Microbiology & Immunology Department, Faculty. of Pharmacy, Ain Shams Univ., Cairo, Egypt | ||||
3-Microbiology Department, Faculty of science, Ain Shams Univ., Cairo, Egypt | ||||
Abstract | ||||
Various virulence factors including invasion, adhesion, cytotoxicity, protease, lipase, elastase, urease production and swarming migration were determined for 24 Proteus isolates recovered from clinical specimens. The results showed that the distribution of virulence factors was different among the test isolates and strain specific in most cases. All Proteus isolates showed invasion and adhesion capabilities with different extents. In addition, they were able to produce elastase, urease and exhibit swarming activity. Protease and lipase activities were not detected in any of the isolates. High cytotoxicity was demonstrated in all isolates. A parallel correlation between invasion and cytotoxicity was demonstrated in all isolates. Five isolates of high virulence factors productivities were selected and identified by Analytical Profile Index as Proteus mirabilis (Pr2, Pr12 and Pr24) and Proteus penneri (Pr6 and Pr20). Plasmid curing by acridine orange resulted in the loss of invasiveness and adhesion capabilities of the five isolates, while other virulence factors levels showed no significant difference changes. The results give a clear evidence that both invasion and adhesion of the tested Proteus isolates are plasmid rather than chromosomally encoded. | ||||
Keywords | ||||
Proteus; Virulence; invasion; adhesion; urease; plasmid and curing | ||||
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