Comparison between culture and non-culture based methods for detection of Nosocomial fungal infections of Candida spp.in intensive care unit patients
Girgis, S., El-Mehalawy, A., Rady, L. (2009). Comparison between culture and non-culture based methods for detection of Nosocomial fungal infections of Candida spp.in intensive care unit patients. EKB Journal Management System, 1(1), 37-47. doi: 10.21608/eajbsg.2009.16716
Samia Abdou Girgis; Adel Ahmed El-Mehalawy; Lamia Mohammed Rady. "Comparison between culture and non-culture based methods for detection of Nosocomial fungal infections of Candida spp.in intensive care unit patients". EKB Journal Management System, 1, 1, 2009, 37-47. doi: 10.21608/eajbsg.2009.16716
Girgis, S., El-Mehalawy, A., Rady, L. (2009). 'Comparison between culture and non-culture based methods for detection of Nosocomial fungal infections of Candida spp.in intensive care unit patients', EKB Journal Management System, 1(1), pp. 37-47. doi: 10.21608/eajbsg.2009.16716
Girgis, S., El-Mehalawy, A., Rady, L. Comparison between culture and non-culture based methods for detection of Nosocomial fungal infections of Candida spp.in intensive care unit patients. EKB Journal Management System, 2009; 1(1): 37-47. doi: 10.21608/eajbsg.2009.16716

Comparison between culture and non-culture based methods for detection of Nosocomial fungal infections of Candida spp.in intensive care unit patients

Egyptian Academic Journal of Biological Sciences, G. Microbiology
Article 5, Volume 1, Issue 1, December 2009, Page 37-47  XML PDF (191.54 K)
Document Type: Original Article
DOI: 10.21608/eajbsg.2009.16716
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Authors
Samia Abdou Girgis1; Adel Ahmed El-Mehalawy2; Lamia Mohammed Rady3
1Clinical pathology Depart. Faculty of medicine, Ain shams university
2Microbiology depart., Faculty of Science, Ain SHAMS UNIVERSITY
3Ain SHAMS SPECIALIZED HOSPITAL, AIN SHAMS UNIVERSITY
Abstract
The main objective of the present study was to compare between culture based method (Hicrome Candida Differential Agar) and non-culture based method (PCR) for detection and identification of Candida spp. (C. albicans, C. glabrata, C. tropicalis and C. parapsilosis) isolated from intensive care unit (ICU) patients of New Kasr El Aini Medical School Educational Hospital, Egypt. The study was carried out during the period August 2008 to September 2009. The results showed that Candida isolates that isolated from Hicrome Candida Differential Agar plates were positive with PCR technique as follow: 100, 95.0, 85.0 and 90.0 for C. albicans, C. glabrata, C. tropicalis and C. parapsilosis, respectively. This means that using of Hicrome Candida Differential Agar media for detection and enumeration of Candida spp. was highly sensitive, rapid and economic method.
Keywords
Nosocomial fungal infections; Candida spp
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