Enhanced Tolerance to Cowpea Mosaic Virus in Vigna unguiculata L. Plants Pretreated with Salicylic Acid | ||||
| Egyptian Journal of Botany | ||||
| Article 2, Volume 62, Issue 2, May 2022, Page 305-318 PDF (1.48 MB) | ||||
| Document Type: Regular issue (Original Article) | ||||
| DOI: 10.21608/ejbo.2021.58315.1610 | ||||
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| Authors | ||||
Ghada Saber Ismail  1; Soad Mohammed Omar2; Faiza Aref Fattouh1
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| 1Botany and Microbiology Department, Faculty of Science, Alexandria University, Alexandria, Egypt | ||||
| 2Botany Department, Faculty of Science, Omar Al-Mukhtar University, Al Bayda, Libya | ||||
| Abstract | ||||
| COWPEA mosaic virus (CPMV) has been identified as an economically critical pathogen infecting cowpea plants. Recently, eco-friendly strategies to enhance the tolerance level of crops against virus infection have been developed. The present investigation evaluates the effect of salicylic acid (SA) on the response of three cultivars of Vigna unguiculata plants [Libyan black eye (LB), Libyan red eye (LR), and Egyptian black eye hybrid (Kafr El Sheikh1cultivar; (EBH)] to CPMV. The leaves of 15-day old cowpea plants were foliar sprayed with 50 or 100 μM SA, 24 h before inoculation with CPMV. Most measured growth indices, total photosynthetic pigment, and photosynthetic efficiency were diminished in CPMV-inoculated plants of all three cultivars. SA utilization could enhance cowpea growth and lower virus severity, most notably in the Libyan cultivars. Furthermore, the CPMV-induced oxidative damages and phenolics accumulation were noted to decrease upon SA application to all infected cowpea cultivars. CPMV infection triggered an increase in catalase (CAT), guaiacol peroxidase (GPX), and polyphenol oxidase (PPO) activity in all cultivars, except for EBH where PPO was insignificantly changed. Interestingly, SA pretreatment was observed to significantly inhibit CAT activity in the Libyan cultivars compared to infected plants. It also induced GPX and PPO in all three tested cultivars, most obviously in the Libyan ones. In general, SA was effective in inducing of systematic resistance to CPMV in the two Libyan cowpea cultivars but not in the Egyptian one. | ||||
| Keywords | ||||
| Antioxidant enzymes; Cowpea mosaic virus; Salicylic acid; Oxidative stress; Phenolic | ||||
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